Lecithin refined

Pluronic F127 and poly (lactic-co-glycolic acid) (PLGA) (50:50, MW: 24,000–38,000 Da) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Lecithin (refined) was supplied by Alfa Aesar (Ward Hill, MA, USA). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol)-2000 (DSPE-PEG₂₀₀₀), folic acid-conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol)-3400 (DSPE-PEG₃₄₀₀-FA) and polystyrene microsphere were obtained from Xi’an Ruixi Corporation (Xi’an, China). Cy5-NHS ester and Cy5 free acid were purchased from Lumiprobe, LLC. (Hallandale Beach, FL, USA). Lut was obtained from Meilun Biotechnology Co. (Dalian, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were provided by HyClone Inc. (Waltham, MA, USA). 4′,6-Diamidino-2-phenylindole (DAPI), and Hydrogen Peroxide Assay Kit (S0038) were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Streptomycin-penicillin solution was supplied by Solarbio Life Sciences Co., Ltd. (Beijing, China). ROS-responsive materials (Oxi-αCD) and Cy5-labeled Oxi-αCD were prepared and characterized as previously reported (Wang et al., 2019 (link); Ni et al., 2020 (link)). The synthesis methods were also listed in the Supporting Information.

Ethanol (Absolute, ≥99.8%, analytical reagent grade) was purchased from Fisher Scientific (UK), Tween 20 and 80 were purchased from Sigma‐Aldrich Ltd (Gillingham, UK), and Lecithin (Refined) was purchased from Alfa Aesar (Lancashire, UK). Sunflower oil (SO) was purchased from the local supermarket. All materials were used with no further purification or modification. The water used in the preparation of all emulsions was passed through a reverse osmosis unit and then a milli‐Q water system.

Unrefined cold-pressed hempseed oil (HSO) was purchased from Manitoba Harvest Hemp Foods (Winnipeg, Manitoba, Canada). Refined lecithin (MW = 758.075 g/mol, Purity > 98.0%) was obtained from Alfa Aesar Co. Inc (Mississauga, Ontario, Canada). Poloxamer 188 (MW ~ 8400 g/mol) was purchased from Corning Co. Inc (Manassas, Virginia, USA). FAME (fatty acid methyl ester) mix containing 14 components provided by Restek Co. (Bellefonte, PA, USA) was used for fatty acid analysis. All other reagents and chemicals were purchased from Sigma-Aldrich Canada Corp (Ontario, Canada) and were analytical or chromatography grade.