Haloperidol was purchased from Tokyo Chemical Industry; acetaminophen and capsaicin from Nacalai Tesque; AM404 from Alomone Labs; methamphetamine from Sumitomo Dainippon Pharma; CNO from Cayman Chemical; DL-2-amino-5-phosphonopentanoic acid (DL-APV) from MilliporeSigma; dinitroquinoxaline-2,3(1H,4H)-dione (DNQX) from Tocris Bioscience (Bio-Techne); bicuculline from Enzo Life Science; and pentobarbital from Kyoritsu Seiyaku.
Haloperidol (1 mg/kg for rats, 2 mg/kg for mice) and acetaminophen (50 or 100 mg/kg for rats, 300 mg/kg for mice) were suspended in 0.5% carboxymethyl cellulose. AM404 was dissolved in 20% dimethyl sulfoxide (DMSO) and diluted with saline. capsaicin was dissolved in DMSO with 1% Tween 80 and diluted in saline (for rats) or 5% DMSO with 1% Tween 80 and diluted in saline (for mice). Methamphetamine was dissolved in saline prior to use. CNO was dissolved in 1% DMSO and diluted in saline for behavioral analysis or in artificial cerebrospinal fluid (ACSF) for electrophysiological analysis.
Haloperidol (1 mg/kg for rats, 2 mg/kg for mice) and acetaminophen (50 or 100 mg/kg for rats, 300 mg/kg for mice) were suspended in 0.5% carboxymethyl cellulose. AM404 was dissolved in 20% dimethyl sulfoxide (DMSO) and diluted with saline. capsaicin was dissolved in DMSO with 1% Tween 80 and diluted in saline (for rats) or 5% DMSO with 1% Tween 80 and diluted in saline (for mice). Methamphetamine was dissolved in saline prior to use. CNO was dissolved in 1% DMSO and diluted in saline for behavioral analysis or in artificial cerebrospinal fluid (ACSF) for electrophysiological analysis.