To construct pCDH-tdTomato expression plasmid, full-length tdTomato was amplified from the pCDH-EF1-Luc2-P2A-tdTomato (Addgene, 72486) with a pair of primers (Forward: 5'-GCTAGCCCAATCATTTAAATATAACTT-3', Reverse: 5'-GCGGCCGCTTACTTGTACAGCTCGTCCATGC-3'), and then cloned into the pCDH-CMV-MCS-EF1α-Neo vector at the Nhe1 and Not1 sites. The sequence of inserted tdTomato was confirmed by DNA sequencing. The constitutively active STAT3 (STAT3-C-Flag) and pCDH-luciferase constructs were generated as previously described7 (link),56 (link). Specific shRNAs against BACE1 (shBACE1; #1: TRCN0000000277, #2: TRCN0000000279) or a non-targeting shRNA (shNT; SHC002) were purchased from Sigma-Aldrich. The lentivirus packaging vectors (ps-PAX2, 12260; and pCI-VSVG, 1733) were from Addgene. The plasmids for expressing human BACE1 with the C-terminal His-tag (HG10064-CH) and human IL-6R with the C-terminal Flag-tag (HG10398-CF) were obtained from Sino Biological.
Hg10398 cf
Manufactured by Sino Biological
The HG10398-CF is a laboratory equipment product offered by Sino Biological. It is designed to perform a core function within the scientific research and testing environment. This product description is presented in a factual and unbiased manner, without any interpretation or extrapolation regarding its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Pcdh cmv mcs ef1α neo vector, by Addgene (2 mentions)
Shbace1 1 trcn0000000277, by Merck Group (2 mentions)
Trcn0000000279, by Merck Group (2 mentions)
Hg10064 ch, by Sino Biological (2 mentions)
Pcdh ef1 luc2 p2a tdtomato, by Addgene (2 mentions)
Pspax2 12260, by Addgene (2 mentions)
Shnt shc002, by Merck Group (2 mentions)
2 protocols using hg10398 cf
1
Construction of pCDH-tdTomato Expression Plasmid
2
Construction of pCDH-tdTomato Expression Plasmid
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To construct pCDH-tdTomato expression plasmid, full-length tdTomato was amplified from the pCDH-EF1-Luc2-P2A-tdTomato (Addgene, 72486) with a pair of primers (Forward: 5'-GCTAGCCCAATCATTTAAATATAACTT-3', Reverse: 5'-GCGGCCGCTTACTTGTACAGCTCGTCCATGC-3'), and then cloned into the pCDH-CMV-MCS-EF1α-Neo vector at the Nhe1 and Not1 sites. The sequence of inserted tdTomato was confirmed by DNA sequencing. The constitutively active STAT3 (STAT3-C-Flag) and pCDH-luciferase constructs were generated as previously described7 (link),56 (link). Specific shRNAs against BACE1 (shBACE1; #1: TRCN0000000277, #2: TRCN0000000279) or a non-targeting shRNA (shNT; SHC002) were purchased from Sigma-Aldrich. The lentivirus packaging vectors (ps-PAX2, 12260; and pCI-VSVG, 1733) were from Addgene. The plasmids for expressing human BACE1 with the C-terminal His-tag (HG10064-CH) and human IL-6R with the C-terminal Flag-tag (HG10398-CF) were obtained from Sino Biological.
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