Cell-attached recordings of CIN activity were performed in male C57 mice (~30-60 days old, Jackson Laboratory) with glass micropipettes (2–4 MΩ) filled with aCSF. Cholinergic interneurons were identified based on their large size, tonic firing, sensitivity to muscarine, and sensitivity to Sk blockade (Yorgason et al., 2017 (link)). Data were filtered at the amplifier with a band-pass Bessel filter 0.3-3 kHz (Axon Instruments Multiclamp 700A; Molecular Devices, San Jose CA). Recordings were sampled continuously at 10 kHz with a PCIe-6321 NI-DAQ board (National Instruments, Austin, TX) and acquired using Axograph 1.7.6 (Axograph Scientific). Recordings were obtained over a 10 minute period, and the last 3 minutes of recordings were used for analysis. The firing rate was analyzed using a simple threshold detection algorithm in Axograph, and focused on measures of instantaneous firing frequency. Values reported are average instantaneous firing frequency over a 3 minute period.
Pcie 6321 ni daq board
Manufactured by National Instruments
The PCIe-6321 NI-DAQ board is a data acquisition device from National Instruments. It features 16 analog input channels, 2 analog output channels, and 24 digital I/O lines. The device utilizes a PCI Express interface for connection to a host computer.
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2 protocols using pcie 6321 ni daq board
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Electrophysiological Recording of Cholinergic Interneurons
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Electrophysiological Recording of Cholinergic Interneurons
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Cell-attached recordings of CIN activity were performed in male C57 mice (~30-60 days old, Jackson Laboratory) with glass micropipettes (2–4 MΩ) filled with aCSF. Cholinergic interneurons were identified based on their large size, tonic firing, sensitivity to muscarine, and sensitivity to Sk blockade (Yorgason et al., 2017 (link)). Data were filtered at the amplifier with a band-pass Bessel filter 0.3-3 kHz (Axon Instruments Multiclamp 700A; Molecular Devices, San Jose CA). Recordings were sampled continuously at 10 kHz with a PCIe-6321 NI-DAQ board (National Instruments, Austin, TX) and acquired using Axograph 1.7.6 (Axograph Scientific). Recordings were obtained over a 10 minute period, and the last 3 minutes of recordings were used for analysis. The firing rate was analyzed using a simple threshold detection algorithm in Axograph, and focused on measures of instantaneous firing frequency. Values reported are average instantaneous firing frequency over a 3 minute period.
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