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Anti mouse cd20

Manufactured by BioLegend

Anti-mouse CD20 is an antibody that specifically binds to the CD20 molecule expressed on the surface of mouse B cells. CD20 is a cell surface marker that is widely used to identify and study B cells in mouse models.

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3 protocols using anti mouse cd20

1

Anti-CD20 Depletion and T Cell Activation

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Mice were injected intravenous with 250 μg of anti-mouse CD20 (clone SA271G2, BioLegend) or with control isotype. Depletion was confirmed by staining of splenic cells with anti-mouse CD19 (BD Biosciences) at day 7. Splenic mononuclear cells were obtained by Ficoll-Hypaque gradients and were cultured in the presence of SEA (20 μg/ml) for 3 days. Supernatants were stored for IL-10 analysis by ELISA.
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2

T Cell Modulation in Transplantation

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On days 2 and 10 after transplantation, 250 μg CTLA4-Ig (Abatacept, Bristol-Myers Squibb) was given by i.p. injection. For the B cell deletion, 250 μg of anti-mouse CD20 (BioLegend, clone SA271G2) was given by i.p. injection on days 0, 7, and 14 after transplantation. For deletion experiments, mice were i.p. administered 0.5 μg of DT in PBS at indicated time points.
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3

T Cell Modulation in Transplantation

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On days 2 and 10 after transplantation, 250 μg CTLA4-Ig (Abatacept, Bristol-Myers Squibb) was given by i.p. injection. For the B cell deletion, 250 μg of anti-mouse CD20 (BioLegend, clone SA271G2) was given by i.p. injection on days 0, 7, and 14 after transplantation. For deletion experiments, mice were i.p. administered 0.5 μg of DT in PBS at indicated time points.
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