A 2PP direct laser writing system (Photonic Professional GT, Nanoscribe) was used to fabricate the micropiston and gripper onto the end of the glass capillary. The outer diameter of the capillary (TSP100170, Polymicro Technologies) without cladding was 140 μm, and the inner diameter was 100 μm. IP-Dip photoresist was used following the same protocol as described in previous work (7 (link)). The main modification to the printing technique was the temporary use of ultraviolet-curable glue to plug the proximal end of the capillary to prevent the photoresist from traveling too far up the hollow channel, thus impeding the removal of the viscous photoresist during the first step of the development in PGMEA for 45 min. To ensure a proper development of the part between the capillary tip and the piston, 2-μm holes were included in the design. These holes were then blocked by the silicone oil and could theoretically withstand a pressure difference two times larger than the pressure difference withstood by the liquid seal between the piston and the cylinder as explained in Eq. 1 . The proximal end of the capillary was then cut and the PGMEA was pumped in through the capillary for 20 min. Isopropyl alcohol was then pumped through the capillary for 5 min to rinse the structure.
Tsp100170
Manufactured by Molex
Sourced in United States
The TSP100170 is a laboratory equipment product manufactured by Molex. It is designed to perform specific functions in a laboratory setting. The core function of this product is to provide reliable and precise measurements or data collection, as required for various laboratory applications. However, a more detailed and unbiased description of the product's features and intended use is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tsp100170
1
Fabrication of Microfluidic Gripper Device
2
Fabrication and Implantation of Microwire Electrode Arrays
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Microwire electrode arrays, each containing 10 stereotrodes (20 channels), were manufactured using formvar-coated nickel-chromium wires with a diameter of 17.78 μm (HFV insulation, California Fine Wire Company). Each stereotrode was threaded through a silica tube (Polymicro Technologies, TSP100170). Each stereotrode was wrapped around two adjacent pins of a standard electrode connector (Omnetics connector, A79026). Silver microwires (OD = 200 μm, 99.95% pure) were then soldered to four pins on the outer side of the connector as ground and reference, respectively. Acrylic resin was used for encapsulation. The electrode tips were plated with platinum to reduce impedance to 300–800 kΩ (at 1 kHz in PBS) before use. The electrode arrays were manufactured as previously described by Sun et al. (2022 (link)).
For electrode implantation, a neural electrode was inserted in the brain at the following stereotaxic coordinates after craniotomy: the tips of the stereotrodes at AP −2.00 mm, ML −1.80 mm, and DV −2.00 mm for dCA3 recording.
For electrode implantation, a neural electrode was inserted in the brain at the following stereotaxic coordinates after craniotomy: the tips of the stereotrodes at AP −2.00 mm, ML −1.80 mm, and DV −2.00 mm for dCA3 recording.
3
Fabrication of Microwire Electrode Arrays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microwire electrode arrays, each containing 10 stereotrodes (20 channels), were fabricated from 17.78 μm diameter formvar-coated nickel-chromium wires (California Fine Wire Company, Grover Beach, CA, United States). Each stereotrode was threaded through a silica tube (TSP100170, Polymicro Technologies, Phoenix, AZ, United States). Each stereotrode was wrapped around two adjacent pins of a standard electrode connector (A79026, Omnetics connector, Minneapolis, MN, United States). Silver microwires (OD = 200 μm, 99.95% pure) were then soldered to four pins on the outer side of the connector as ground and reference, respectively. Acrylic resin was used for the encapsulation. Electrode tips were plated with platinum to reduce impedance to 300–800 kΩ (at 1 kHz in PBS) before use. The electrode arrays were fabricated as previously described by Sun et al. (2022) (link).
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