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Hydrogen tetrachloroaurate trihydrate haucl4 3h2o

Manufactured by Merck Group
Sourced in United States

Hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O) is a chemical compound used in various laboratory applications. It serves as a precursor for the synthesis of gold nanoparticles and can be used in the deposition of thin gold films. The compound is a yellow crystalline solid that is soluble in water and common organic solvents.

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4 protocols using hydrogen tetrachloroaurate trihydrate haucl4 3h2o

1

Synthesis of Diverse Nanoparticles

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The following chemicals
were used: TEOS (99%, Sigma-Aldrich), l-arginine (98%, Sigma-Aldrich),
absolute ethanol (Merck), MilliQ water (Millipore system), sodium
hydroxide (reagent grade, ≥98%, pellets, Sigma-Aldrich), hydrogen
tetrachloroaurate trihydrate (HAuCl4·3H2O, ≥99.9%, Sigma-Aldrich), sodium citrate tribasic dihydrate
(≥99.0%, Sigma-Aldrich), polyvinylpyrrolidone (PVP, 10000 g/mol),
polyoxyethylene (5) nonylphenyl ether (IGEPAL CO-520, Sigma-Aldrich),
ammonia (NH3, 30 wt %, Sigma-Aldrich), iron chloride (FeCl3·6H2O, 98%, Sigma- Aldrich), sodium oleate
(95%, TCL), hexane (98%, Sigma-Aldrich), oleic acid (90%, Sigma-Aldrich),
1-octadecene (90%, Sigma-Aldrich), and potassium persulfate (K2S2O8, ≥99%, Sigma-Aldrich).
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2

Synthesis of Gold-Peptide Nanoparticles

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All chemicals were commercially available and used without further purification. Hydrogen tetrachloroaurate trihydrate (HAuCl4.3H2O) and GSH were obtained from Sigma-Aldrich. Custom-designed oligoarginine peptide CRRRRRRRRR (CR9) was supplied by GL Biochem Ltd. (Shanghai, China). Ultrapure water (Milli-Q) with a resistivity of 18.2 MΩ was used as the general solvent throughout the study.
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3

Gold Nanoparticle Synthesis and Characterization

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Glutathione (GSH, γ-Glu-Cys-Gly) and human serum albumin (HSA) were obtained from Sigma-Aldrich (purity: 95%, St. Louis, MO, USA); hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O) was also purchased from Sigma-Aldrich. Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) and fetal bovine serum (FBS) were purchased from Gibco (Waltham, MA, USA). The CCK-8 kit (Cell Counting Kit 8) was acquired from Dojindo Laboratories (Kumamoto, Japan). The recombinant Mouse IFN gamma (IFN-γ) protein was obtained from Novoprotein (Suzhou, China). The enzyme-linked immunosorbent assay (ELISA) assay kits for IFN-γ, IL-6, and TNF-α were obtained from Mreda Company (Beijing, China). Antibodies against p-STAT1/STAT1, STAT3, β-actin, and HRP-labeled Goat Anti-Rabbit IgG (H + L) second antibody were purchased from Beyotime Biotechnology (Shanghai, China). Antibodies against p-STAT3, Caspase3, p-JAK2, and JAK2 were purchased from Bioss (Beijing, China).
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4

Synthesis and Characterization of Gold Nanoparticles

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All chemicals were purchased from commercial suppliers and used without further purification. CTAB (Bioxtra, ≥99.0%), BDAC (cationic detergent), cetyltrimethylammonium chloride, citric acid trisodium salt dihydrate (≥99.5%, BioUltra, for molecular biology) (CTAC, ≥98.0%), hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O) and L-ascorbic Acid (Bioxtra, ≥98.0%) were purchased from Sigma Aldrich. Silver nitrate (AgNO3, ≥99.8%) and sodium borohydride (NaBH4, ≥99%) were purchased from Fluka. Hydrochloric acid (HCl, 1 N) was purchased from Fisher scientific. Nanopure water (18.2 MΩ, Barnstead Nanopure, Thermo Scientific, MA, USA) was used in all experiments. The glass vials were purchased from Kimble chase (4 and 20 ml, NJ, USA). All glasswares were cleaned using freshly prepared aqua regia (HCl:HNO3 in a 3:1 ratio by volume) followed by rinsing with copious amounts of water. RCT Basic (IKA, NC, US) was used for magnetic stirring. Ultraviolet–vis–near-infrared spectra were measured with Synergy H4 (Biotek, VT, USA) and Cary 5000 ultraviolet–vis–near-infrared (Agilent, CA, USA). The formvar/carbon-coated copper grid (Ted Pella, Inc. Redding, CA, USA) and TEM (Tecnai G2 F30 Super Twin microscope, 300 kV and Tecnai G2 Spirit, 200 kV, FEI, OR, USA) were used for the TEM analysis.
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