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2 protocols using kasumi 1 cells

1

Cell Culture Protocol for Kasumi-1, HEK 293T, and KO52

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Kasumi-1 and HEK 293T cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen. KO52 were obtained from the Japanese Collection of Research Bioresources Cell Bank. Kasumi-1 cells were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) (Gibco), 5% Penicillin-Streptomycin (Pen-Strep) (Sigma-Aldrich), 5% L-Glutamine (L-Glu) (Sigma-Aldrich). HEK 293T cells were cultured in DMEM supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu. KO52 were cultured in Alpha MEM (Lonza) supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu.
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2

Cell Culture Conditions for Kasumi-1, HEK293T, and KO52 Cells

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Kasumi-1 and HEK293T cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen. KO52 were obtained from the Japanese Collection of Research Bioresources Cell Bank. Kasumi-1 cells were cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% fetal calf serum (FCS) (Gibco), 5% Penicillin-Streptomycin (Pen-Strep) (Sigma-Aldrich), 5% L-Glutamine (L-Glu) (Sigma-Aldrich). HEK 293T cells were cultured in DMEM supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu. KO52 were cultured in Alpha MEM (Lonza) supplemented with 10% FCS (Gibco), 5% Pen-Strep, 5% L-Glu.
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