Ts1 8

Manufactured by BioLegend

The TS1/8 is a laboratory equipment product manufactured by BioLegend. It is a specialized device designed for specific applications in research and scientific settings. The core function of the TS1/8 is to perform a particular task or operation, but a detailed and unbiased description without extrapolation or interpretation is not available at this time.

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Lab products found in correlation

Accutase, by STEMCELL (1 mentions) Clone 12g5, by BioLegend (1 mentions) Efluor 780, by Thermo Fisher Scientific (1 mentions) Pan monocyte isolation kit, by Miltenyi Biotec (1 mentions) Opteia, by BD (1 mentions) Hit3a, by BioLegend (1 mentions)

2 protocols using ts1 8

Flow Cytometric Analysis of CXCR4 and CD2

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Cell were plated in a 24-well plate and guide transfection and heat treatment were done as abovementioned. One day after heat treatment, cells were harvested by adding 100 μL Accutase (STEMCELL technologies) and transferred to a 1.5 mL tube. Cells were centrifuged for 2 min to remove supernatant and then resuspended in 375 μL blocking buffer containing 10% FBS in PBS. Cells were centrifuged again to remove the blocking buffer and resuspended in 100 μL blocking buffer containing APC-CXCR4 (BioLegend, Clone 12G5, Cat # 306510, 1:500 dilution) or APC-CD2 (BioLegend, TS1/8, Cat # 309224, 1:50 dilution). Cells were incubated with antibodies (1 hr at R.T. for CXCR4 and 30 min 4 °C for CD2), protected from light. To remove the antibody and wash, cells were pelleted by centrifuging and resuspended in 375 μL blocking buffer. Cells were centrifuged again and resuspended in 150 μL blocking buffer and immediately analyzed by flow cytometry.

Liu P., Foiret J., Situ Y., Zhang N., Kare A.J., Wu B., Raie M.N., Ferrara K.W, & Qi L.S. (2023). Sonogenetic control of multiplexed genome regulation and base editing. Nature Communications, 14, 6575.

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Quantifying Cytokine Production in T Cells

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Primary T cells were stimulated for 4 h at 37 °C by plate-bound anti-human CD3 antibody (HIT3a, BioLegend). APC anti-human TNF antibody (1:200, Mab11, BioLegend) was added to the cells during stimulation. Cells were collected, washed with FACS buffer (2% FBS and 0.05% NaN₃ in PBS) and stained for Pacific Blue or BV421 anti-human CD2 antibody (TS1/8, BioLegend) and fixable viability dye eFluor780 (1:1,500, eBioscience). Cells were fixed with 2% paraformaldehyde for 15 min and analyzed by flow cytometry. For iRHOM2-overexpressed cells, anti-EGFR antibody (1:200, R&D Systems) was used to gate transduced cells. Cell culture supernatants were collected following stimulation, and TNF was measured using the human TNF ELISA Set (BD OptEIA, BD Biosciences). CD14⁺ monocytes were isolated from PBMCs using the Miltenyi Pan Monocyte Isolation Kit and then stimulated for 4 h with 125 ng ml⁻¹ LPS cRPMI. mTNF levels were measured as described above.

Kubo S., Fritz J.M., Raquer-McKay H.M., Kataria R., Vujkovic-Cvijin I., Al-Shaibi A., Yao Y., Zheng L., Zou J., Waldman A.D., Jing X., Farley T.K., Park A.Y., Oler A.J., Charles A.K., Makhlouf M., AbouMoussa E.H., Hasnah R., Saraiva L.R., Ganesan S., Al-Subaiey A.A., Matthews H., Flano E., Lee H.H., Freeman A.F., Sefer A.P., Sayar E., Çakır E., Karakoc-Aydiner E., Baris S., Belkaid Y., Ozen A., Lo B, & Lenardo M.J. (2021). Congenital iRHOM2 deficiency causes ADAM17 dysfunction and environmentally directed immunodysregulatory disease. Nature immunology, 23(1), 75-85.

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