Gms10014

The activities of CS, CPT-1 and COX were examined using a CS assay kit (GMS50130.1; Genmed Scientifics, Inc., Shanghai, China), a CPT-1 assay kit (GMS50118.1.2; Genmed Scientifics, Inc.) and a COX assay kit (GMS10014.2; Genmed Scientifics, Inc.), respectively, according to the manufacturers' instructions.

PC12 pheochromocytoma cells were obtained from ATCC (Rockville, MD, USA), and paeoniflorin was purchased from Winherb Medical Science Co., Ltd. (purity: 98.96%, 23180-57-6, Shanghai, China). Bortezomib was purchased from Meilun Biotechnology Co., Ltd. (MB1040, Dalian, China), and a Cell Counting Kit-8 (CCK-8) was purchased from Servicebio Technology Co., Ltd. (G4103, Wuhan, China). An apoptosis kit was obtained from Keygen Biotech Co., Ltd. (KGA108, Nanjing, China), and a living cell mitochondrial damage/oxidation (NAO) fluorescence assay kit was from GenMed (GMS10017.1, USA). Antibodies against LC3, p62, Beclin-1, Pink1, and Parkin were purchased from Santa Cruz Biotechnology (USA). An intelligent hot plate was obtained from Zhenghua Biologic Apparatus Facilities (ZH-YLS-6BS, Anhui, China), and a Sting thermal imager was obtained from Taimeng Software Co., Ltd. (PL-200, Chengdu, China). A multichannel physiological recorder was obtained from Biopac (MP160, USA), and a monoamine oxidase activity colorimetric assay kit (GMS50022.1) and purified mitochondrial cytochrome c oxidase activity assay kit (GMS10014.2) were purchased from GenMed (Shanghai, China).