Soxtec 2055 fat extraction system

Manufactured by Foss

Sourced in Sweden, Denmark

The Soxtec™ 2055 Fat Extraction System is a laboratory equipment designed for the rapid and efficient extraction of fat content from various sample types. The system utilizes a standardized Soxhlet extraction method to determine the fat or oil content of a sample.

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Lab products found in correlation

Cr 410, by Konica Minolta (1 mentions) Breeze software, by Waters Corporation (1 mentions)

Spelling variants of this product

Soxtec 2055 (15 citations) Soxtec Avanti 2055 Fat Extraction System (3 citations) Extraction System 2055 (2 citations)

4 protocols using soxtec 2055 fat extraction system

Isoenergetic Dietary Protein-Carbohydrate Ratios

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The experimental diets were isoenergetic (21 kJ/g DM), isolipidic (6 % DM) and contained four different dietary crude protein:total CH ratios: 48:38, 52:34, 56:30 and 60:26. The formulation and proximate composition of diets (Sparos) are presented in Table 1.
Diet composition analysis was carried out in duplicate following the methodology described by the Association of Official Analytical Chemists (AOAC) (33) . Ash was analysed by combustion (550°C during 6 h) in a muffle furnace (Nabertherm L9/11/B170) and crude protein (N × 6•25) using a Leco N analyser (model FP-528; Leco Corporation). Crude lipid content was determined by petroleum ether extraction (40-60°C) using a Soxtec™ 2055 Fat Extraction System (Foss), whereas starch followed Thivend et al. (34) . Gross energy was quantified in an adiabatic bomb calorimeter (Werke C 2000 basic; IKA).

Conde-Sieira M., Salas-Leiton E., Duarte M.M., Pelusio N.F., Soengas J.L, & Valente L.M. (2016). Short- and long-term metabolic responses to diets with different protein:carbohydrate ratios in Senegalese sole (Solea senegalensis, Kaup 1858). The British journal of nutrition, 115(11).

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Intramuscular Fat Content Measurement

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The longissimus muscle samples were lyophilized and ground to powders. The IMF content was measured by petroleum ether (30 to 60°C boiling point) extraction using the Soxtec 2055 fat extraction system (Foss Tecator AB, Sweden), according to the Association of Official Analytical Chemists method [33 ].

Ma X., Zheng C., Hu Y., Wang L., Yang X, & Jiang Z. (2015). Dietary L-Arginine Supplementation Affects the Skeletal Longissimus Muscle Proteome in Finishing Pigs. PLoS ONE, 10(1), e0117294.

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Meat Quality Analysis Protocol

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The pH of muscle samples was measured at 45 min, 24 h, and 48 h postmortem using a pH meter (HI 8242C, Beijing Hanna Instruments Science & Technology, Beijing, China). Meat color CIE LAB values (L*, lightness; a, redness; b, yellowness) were determined on the transverse surface of the LM after it was cut and let to bloom for 45 min at the same times postmortem using a colorimeter (CR-410, Minolta, Suita-shi, Osaka, Japan), as described by Mason et al. [12 (link)]. Shear force was measured using an Instron Universal Mechanical Machine (Instron model 4411; Instron, Canton, MA, USA) and drip loss was measured by weight loss over 24 h at 4 °C in a plastic bag, also as described by Mason et al. [12 (link)]. The IMF content was measured by petroleum ether extraction of powdered, lyophilized muscle using the Soxtec 2055 fat extraction system (Foss Tecator AB, Höganäs, Sweden), according to the Association of Official Analytical Chemists method [13 ].

Ma X., Wang L., Shi Z., Chen W., Yang X., Hu Y., Zheng C, & Jiang Z. (2019). Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes & Nutrition, 14, 23.

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Comprehensive Chemical Analysis of Aquatic Samples

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Analysis of diets, whole fish, and feces followed the methodology described by AOAC [16 ]. Dry matter after drying at 105 °C for 24 h; total ash by combustion (550 °C during 6 h) in a muffle furnace (Nabertherm L9/11/B170, Lilienthal, Germany); crude protein (Nx6.25) by a flash combustion technique, followed by a gas chromatographic separation and thermal conductivity detection with a Leco N Analyzer (Model FP-528, Leco Corporation, Benton Harbor, MI, USA); crude lipid by petroleum ether extraction (40–60 °C) using a Soxtec™ 2055 Fat Extraction System (Foss, Hillerød, Denmark), with prior acid hydrolysis with 8.3 M HCl; gross energy in an adiabatic bomb calorimeter (Werke C2000, IKA, Hohenems, Germany). Chromium concentrations in feeds and feces were determined according to Bolin et al. [17 (link)], after perchloric acid digestion. Amino acids were determined after hydrolysis in 6M HCL at 108 °C for 24 h in nitrogen-flushed glass vials. A Waters Pico-Tag reversed-phase HPLC system using norleucine as an internal standard was used. The resulting chromatograms were analyzed with Breeze software (Waters, Milford, MA, USA). The tryptophan in complete feeds was analyzed according to ISO 13904:2016 by HPLC-FD methodology [18 ].

do Vale Pereira G., Teixeira C., Couto J., Dias J., Rema P, & Gonçalves A.T. (2024). Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia. Animals : an Open Access Journal from MDPI, 14(5), 714.

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