Cells were lysed by ice-cold RIPA buffer (Boston BioProducts) containing protease inhibitors (Roche, Sigma) and incubated on ice for 30 mins. Protein supernatant was collected after centrifuging at 13,000g 4 °C for 30 mins. Protein concentration was determined using the Bradford protein assay (Bio-Rad). Protein samples were separated under reducing conditions on 4–15% Tris-HCl gels (Bio-Rad) and analyzed by western blotting using primary antibodies: mouse anti-LbCpf1 (Diagenode 1:3000) followed by secondary anti-rabbit HRP antibodies (Sigma-Aldrich, 1:10,000). Blots were imaged with an Amersham Imager 600.
Anti rabbit hrp antibodies
Manufactured by Merck Group
Anti-rabbit HRP antibodies are laboratory reagents used to detect and quantify the presence of rabbit-derived proteins or antigens in samples. The antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that catalyzes a colorimetric reaction, enabling the visualization and measurement of target rabbit proteins.
Automatically generated - may contain errors
2 protocols using anti rabbit hrp antibodies
1
LbCpf1 Protein Analysis by Western Blot
2
LbCpf1 Protein Analysis by Western Blot
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Cells were lysed by ice-cold RIPA buffer (Boston BioProducts) containing protease inhibitors (Roche, Sigma) and incubated on ice for 30 mins. Protein supernatant was collected after centrifuging at 13,000g 4 °C for 30 mins. Protein concentration was determined using the Bradford protein assay (Bio-Rad). Protein samples were separated under reducing conditions on 4–15% Tris-HCl gels (Bio-Rad) and analyzed by western blotting using primary antibodies: mouse anti-LbCpf1 (Diagenode 1:3000) followed by secondary anti-rabbit HRP antibodies (Sigma-Aldrich, 1:10,000). Blots were imaged with an Amersham Imager 600.
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