G morpholinepropanesulfonic acid mops medium

ICSI was performed as previously described25 (link) with some changes. The wife of the patient underwent controlled ovarian hyperstimulation with a standard long protocol. For oocyte maturation, human chorionic gonadotropin (hCG) was used. Oocyte handling was performed using G-morpholinepropanesulfonic acid (MOPS) medium (Vitrolife, Kungsbacka, Sweden). Oocyte denudation was performed with hyaluronidase using G-in vitro fertilization (IVF) PLUS medium (Vitrolife). Embryo culture up to day 3 was performed with G-1 medium (Vitrolife) in a desktop incubator (COOK Medical, Bloomington, IN, USA). For luteal support, progesterone was used. Clinical pregnancy was confirmed via two hCG tests conducted in 14 days following embryo transfer, where clinical pregnancy was defined as at least a visible sac detected via ultrasound screening after 30 days in Reproductive and Genetic Hospital at The First Affiliated Hospital of University of Science and Technology of China. The datasets of variants for this study can be found in the NCBI dbVar database (SUB7725323).