Premix Primer STAR HS was purchased from Takara Bio Inc. (Otsu, Japan), agarose powder was purchased from Biowest (Miami, FL, USA), loading buffer was purchased from Takara Bio, Inc., GoldenView nucleic acid dyes were purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd. (Beijing, China) and EZNA Blood DNA kit was purchased from Omega Bio-Tek Inc. (Norcross, GA, USA).
Agarose powder
Manufactured by Biowest
Sourced in Spain
Agarose powder is a purified polysaccharide derived from red seaweed. It is a commonly used material in biotechnology and molecular biology applications. The primary function of agarose powder is to serve as a gelling agent in the preparation of agel matrices for the separation and analysis of macromolecules, such as DNA, RNA, and proteins.
Automatically generated - may contain errors
Lab products found in correlation
Alginate, by Merck Group (3 mentions)
Gfp huvec, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Loading buffer, by Takara Bio (1 mentions)
E z n a blood dna kit, by Omega Bio-Tek (1 mentions)
Alginate powder, by Merck Group (1 mentions)
Celltracker red, by Thermo Fisher Scientific (1 mentions)
Sylgard 184, by Dow (1 mentions)
Rpmi 1640 medium, by Solarbio (1 mentions)
Sulfosuccinimidyl 4 n maleimidomethyl cyclohexane 1 carboxylate sulfo smcc, by Merck Group (1 mentions)
Safranin o fast green, by Wuhan Servicebio Technology (1 mentions)
Hematoxylin and eosin h e, by Wuhan Servicebio Technology (1 mentions)
Dna ladder, by Solarbio (1 mentions)
Enzyme linked immunosorbent assay elisa kits, by MultiSciences Biotech (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Complete freund s adjuvant cfa, by Chondrex (1 mentions)
Caspase 3 activity assay kit, by Beyotime (1 mentions)
5 protocols using agarose powder
1
Extraction and Amplification of Blood DNA
2
Agarose-Alginate Hydrogel Fabrication
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Alginate with medium viscosity (2000 mPa·s) was purchased from Sigma (United Kingdom). Calcium chloride powder was bought from Aladdin (Shanghai, China). Agarose powder with low melting temperature (87–89 °C) was bought from Biowest (Spain). 3% (w/ v) Alginate solution was prepared by dissolving Alginate powder into phosphate buffer saline (PBS). 2% (w/v) agarose solution with 3% (w/v) calcium chloride was prepared by dissolving agarose and calcium chloride powders in tris-buffered saline (TBS) at 100 °C. 1% (w/ v) calcium chloride solution was prepared by dissolving calcium chloride powder into TBS at room temperature. Flat agarose hydrogel with a thickness of 3 mm was prepared by casting agarose solution in a petri dish. For electrohydrodynamic cell printing, rat myocardial cell lines (H9C2, ATCC) were added into Alginate solution with a density of 1×106 cells mL-1.
3
Tissue Engineering Scaffold Fabrication
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Type I collagen derived from rat tail was prepared followed by previously described protocols and stored at 4°C. CaCl2 and sodium hydroxide (NaOH) powder were purchased from Aladdin (Shanghai, China). Agarose powder with low melting temperature (87–89°C) was bought from Biowest (Spain). Alginate solution with a viscosity of 2000 mPa·S was prepared by dissolving Alginate powder (Sigma, United Kingdom) into the cell culture medium with 10% (w/v) fetal bovine serum and 1% (w/v) antibiotic/antimycotic. 0.1 mol/L NaOH solution was prepared by dissolving NaOH powder into phosphate buffer saline (PBS) at room temperature. 3% (w/v) CaCl2 solution was prepared by dissolving CaCl2 powder into tris-buffered saline (TBS) at room temperature. 2% (w/v) flat agarose hydrogel was prepared by casting the boiling agarose solution with 3% (w/v) CaCl2 into a petri dish at room temperature. Before the printing process, the pH of the collagen solution was adjusted to 7.2-7.6. GFP expressing human umbilical vein endothelial cells (GFP-HUVEC; ATCC, USA) and embryonic rat cardiomyocytes (H9C2; ATCC, USA) labeled with red cell tracker (Invitrogen, cat. no. C34552) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo) for printing cell-laden constructs.
4
Hydrogel-based Cell Printing Technique
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Polydimethylsiloxane (PDMS, Sylgard 184) was obtained from Dow Corning (Midland, MI, USA). Alginate with medium viscosity was purchased from Sigma Aldrich (St. Louis, MO, USA). Calcium chloride powder was bought from Aladdin (Shanghai, China). Agarose powder with low melting temperature (87–89°C) was bought from Biowest (Spain). Green fluorescent particles with a particle size of 10 µm and red fluorescent particles with particle size of 10 µm and 3.2 µm were purchased from Base Line (Tianjin, China). Red/green/blue/yellow pigment was purchased from M and G (China). Alginate solutions with different concentration of 1%, 2%, and 3% (w/v) were prepared by dissolving Alginate powder into distilled water or culture medium. About 2% (w/v) agarose solution with 2% (w/v) calcium chloride was prepared by dissolving agarose and calcium chloride powders into distilled water at 100°C. After boiling, agarose solution was poured in a Petri dish and cooled down to form a flat agarose hydrogel with a thickness of 3 mm as the collecting substrate. For cell printing, GFP expressing human umbilical vein endothelial cells (GFP-HUVEC; ATCC, Manassas, VA, USA) and red fluorescent protein embryonic rat cardiomyocytes (H9C2, ATCC, Manassas, VA, USA) were added into 3% Alginate solution with a density of 5 × 105 cells mL−1.
5
Synthesis and Characterization of Compounds for Rheumatoid Arthritis Study
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Oligonucleotides and peptide were synthesized by Sangon Biotech (Shanghai, China) and Huajin Biotechnology (Shanghai, China). Agarose powder was obtained from Biowest (Nuaillé, France). Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-SMCC) was supplied by Sigma (Darmstadt, Germany). EN was purchased from Shanghai CP Guojian Pharmaceutical (Shanghai, China). Cell counting kit 8 (CCK8), annexin V-FITC apoptosis staining/detection kit and caspase 3 activity assay kit were purchased from Beyotime Biotechnology (Shanghai, China). RPMI Medium 1640 was obtained from Beijing Solarbio Science & Technology (Beijing, China). Fetal bovine serum (FBS) was provided by BI (Kibbutz, Israel). Complete Freund's adjuvant (CFA) and immune grade chick type II Collagen were purchased from Chondrex (Woodinville, USA). Freund's adjuvant incomplete (IFA) was purchased from Sigma (Saint Louis, USA). Enzyme linked immunosorbent assay (ELISA) kits were purchased from MultiSciences Biotech (Hangzhou, China). DNA Ladder and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Beijing Solarbio Science & Technology (Beijing, China). Hematoxylin and eosin (H&E) and Safranin O-fast green were provided from Servicebio (Wuhan, China). All chemicals and materials were used as received without any further modifications.
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