Mimics of miR-483-3p (483-3p-M) and miR-483-5p (483-5p-M), inhibitors of miR-483-3p (483-3p-I), and their corresponding negative controls (mimic-NC (M-CT) and inhibitor-NC (I-CT)), and the siRNA of IGF1 (si-IGF1) and its corresponding negative control (si-NC) were synthesized by Genepharma (Shanghai, China). hADSCs were grown until 50%–60% confluence then transfected with 100 nM miRNA mimic, inhibitor or NC using Lipofectamine 2000 reagent (Invitrogen, CA, USA). The culture medium was changed after 12 hours of transfection and cellular differentiation was induced 48 hours later according to the manufacturer’s recommendations.
Si igf1
Manufactured by GenePharma
Sourced in China
Si-IGF1 is a laboratory equipment product designed for research purposes. It is used to measure the levels of insulin-like growth factor 1 (IGF-1) in biological samples. The core function of Si-IGF1 is to provide accurate and reliable quantification of IGF-1 concentration, which is a key indicator of various physiological and pathological processes.
Automatically generated - may contain errors
Lab products found in correlation
Si nc, by GenePharma (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Si zeb1, by GenePharma (1 mentions)
Oe zeb1, by GenePharma (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Mir 497 mimics, by GenePharma (1 mentions)
Si hcp5, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Lentivirus particles, by GenePharma (1 mentions)
3 protocols using si igf1
1
Modulating miR-483 and IGF1 in hADSCs
2
IGF-1 and ZEB1 Regulation in HTR-8/SVneo Cells
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HTR‐8/SVneo cells were transfected with plasmids containing transcripts of si‐IGF‐1, overexpressed (oe)‐IGF‐1, si‐ZEB1 or oe‐ZEB1, respectively (plasmids were all purchased from GenePharma,). Transfection was conducted according to the manufacturer's instructions of Lipofectamine™ 2000 Transfection Reagent (11,668,019, Invitrogen), and their expressions were assessed by performing RT‐qPCR or Western blot analysis after 48 h of transfection.
3
Lentivirus-mediated Gene Regulation
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Lentivirus particles expressing sh-HCP5, pcDNA3.1 HCP5, si-HCP5, miR-497 mimics, si-IGF1, as well as their negative control were synthesized and purchased from GenePharma (Shanghai, China). The transfection was performed using Lipofectamine 3000 (Invitrogen, CA, USA) as the manufacturer's guidance described. Briefly, cells were grown up to 70-80% confluence and then 1 μg plasmid together with 1 μL Lipofectamine 3000 were added into the culture medium.
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