60f245 plates

All chemicals were obtained from Thermo Fisher Scientific (Seoul, Korea), SEJIN CI Co. (Seoul, Korea), or Sigma-Aldrich (St. Louis, MO, USA). Reagents purchased were used without further purification. Solvents requiring anhydrous conditions were distilled over CaH₂, or Na and benzophenone before use. Reactions were carried out in a nitrogen atmosphere and analyzed by thin layer chromatography (TLC) using pre-coated 60F₂₄₅ plates purchased from Merck. Flash column chromatography using MP Silica 40–63, 60 Å was performed. Subsequently, ¹H, ¹³C, and ¹⁹F NMR were measured at 500, 125, and 470 MHz, respectively. Mass data for low resolution were recorded in ESI modes (positive and negative) using an Expression CMS mass spectrometer (Advion Ithaca, NY, USA). CDCl₃, CD₃OD, and DMSO-d₆ were used as solvents for NMR. All chemical shifts were measured in ppm (parts per million) versus residual solvent or deuterated peaks (δ_H 7.26, δ_H 3.31, and δ_H 2.48 for CDCl₃, CD₃OD, and DMSO-d₆, respectively, and δ_C 77.0, δ_C 49.0, and δ_C 40.0 for CDCl₃, CD₃OD, and DMSO-d₆, respectively). Coupling constants (J) are represented in hertz (Hz). The following abbreviations for ¹H NMR were used: dd (doublet of doublets), brs (broad singlet), s (singlet), q (quartet), t (triplet), and d (doublet).

Reagents and chemicals were obtained commercially and used without further purification. All reactions were monitored by thin layer chromatography (TLC) using Merck precoated 60F₂₄₅ plates, and reaction mixtures were purified by column chromatography using MP Silica 40–63 (60 Å). Mass spectroscopy was performed in electrospray ionization (ESI) positive mode using an Expression CMA spectrometer (Advion Ithaca, NY, USA). ¹H NMR (400 and 500 MHz) and ¹³C NMR (100 and 125 MHz) data were obtained using a Varian Unity INOVA 400 spectrometer or a Varian Unity AS500 spectrometer (Agilent Technologies, Santa Clara, CA, USA). DMSO‑d₆ and CDCl₃ were used as NMR solvents. Chemical shift values were recorded in parts per million (ppm) and coupling constants (J) were recorded in hertz (Hz). The following abbreviations are used: s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), and brs (broad singlet).