Cellsens dimension software ver 1

We observed cells through an Olympus FLUOVIEW FV3000 confocal laser scanning microscope (Olympus). Image acquisition and processing, including deconvolution and signal quantitation, were performed by Acquisition FV31S-SW (Olympus), Analysis FV31S-DT (Olympus), and cellSens Dimension software Ver. 1.18 (Olympus). Alternatively, we observed cells through a DM IRE2 motorized fluorescence microscope (Leica) equipped with an ARC LAMP power supply HBO100 DC IGN (Ludl Electronic Products, Ltd.), and an ORCA-ER high-resolution digital CCD camera (Hamamatsu). Image acquisition and processing, including deconvolution and signal quantitation, were performed by Openlab version 5.5.2 Scientific Imaging Software (Improvision) and Velocity version 6.3 3D Image Analysis Software (Improvision).
Quantitation of centromere signal intensity in interphase cells was performed manually as described (Niikura and Kitagawa, 2016 (link); Niikura et al., 2016 (link); Niikura et al., 2015 (link); Niikura et al., 2017 (link)) (see QUANTIFICATION AND STATISTICAL ANALYSIS), or similar procedures were applied by cellSens Dimension software Ver. 1.18 (Olympus). The integrated signal intensity was calculated by subtracting the fluorescence intensity of the background (measured outside the nucleus). Deconvolved and max-projected images were used for fluorescence intensity measurements.