Anti par antibody

Manufactured by R&D Systems

The Anti-PAR antibody is a laboratory reagent used to detect and quantify Poly(ADP-Ribose) (PAR) in biological samples. PAR is a post-translational modification involved in various cellular processes. The Anti-PAR antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to study the role of PAR in biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti parp2 antibody, by Active Motif (2 mentions) Anti α tubulin antibody, by Merck Group (2 mentions) Niraparib, by Selleck Chemicals (1 mentions) Talazoparib, by Selleck Chemicals (1 mentions) Anti histone h3 antibody, by Abcam (1 mentions) Anti β actin antibody, by Merck Group (1 mentions) 8 mop, by Merck Group (1 mentions) Olaparib, by Selleck Chemicals (1 mentions) Anti parp1 antibody, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions)

2 protocols using anti par antibody

PARP Inhibitor Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PARP inhibitors olaparib (Selleckchem, S1060), niraparib (Selleckchem, S2741), and talazoparib (Selleckchem, S7048) were dissolved in dimethyl sulfoxide (DMSO) and used at 1 μM final concentration. Anti-PARP1 antibody (CST, 9542) was used at 1:5000. Anti-PARP2 antibody (Active Motif, 39044) was used at 1:2000. Anti-XRCC1 antibody (Novus Biologicals, 87154) was used at 1:5000. Anti-PAR antibody (R&D, 4335-MC-100) was used at 1:1000. Anti-Histone H3 antibody (Abcam, ab1791) was used at 1:5000. Anti-α-Tubulin antibody (Sigma, CP06) was used at 1:5000. Anti-β-Actin antibody (Sigma, A5441) was used at 1:10 000. 8-MOP (Methoxsalen) (Sigma, M3501) was added 10 min before micro-irradiation at a final concentration of 100 μM when used.

Lin X., Jiang W., Rudolph J., Lee B.J., Luger K, & Zha S. (2022). PARP inhibitors trap PARP2 and alter the mode of recruitment of PARP2 at DNA damage sites. Nucleic Acids Research, 50(7), 3958-3973.

+ Open protocol

+ Expand

Western Blot Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used in this study for western blot include: anti-PARP1 antibody (Cell Signaling Technology, 9542, 1:2,000), anti-PARP2 antibody (Active Motif, 39044, 1:2,000), anti-α-Tubulin antibody (Sigma, CP06, 1:1,000), anti-pan-ADP-ribose binding reagent (Sigma, MABE1016, 1:1,000), anti-β-actin (Sigma, A5441, 1:10,000), and anti-PAR antibody (R&D, 4335-MC-100, 1:1,000).

Shao Z., Lee B.J., Zhang H., Lin X., Li C., Jiang W., Chirathivat N., Gershik S., Shen M.M., Baer R, & Zha S. (2023). Inactive PARP1 causes embryonic lethality and genome instability in a dominant-negative manner. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2301972120.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!