Sgii rabbit antibody

Manufactured by Meridian Bioscience

Sourced in United States

The SgII rabbit antibody is a laboratory reagent used in scientific research. It is a specific antibody raised in rabbits that recognizes the SgII protein, which is a member of the secretogranin family of proteins. The antibody can be used to detect and study the presence and distribution of SgII in biological samples through techniques such as immunohistochemistry or Western blotting. The core function of this product is to provide a tool for researchers to investigate the expression and localization of SgII in their areas of study.

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Lab products found in correlation

Ha 3f10 rat mab, by Roche (2 mentions) Tgn38 mouse mab, by BD (2 mentions) Hid 1 mouse mab, by Novus Biologicals (2 mentions) Anti mouse alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit alexafluor647, by Thermo Fisher Scientific (1 mentions) Anti mouse alexa fluor 647, by Thermo Fisher Scientific (1 mentions)

2 protocols using sgii rabbit antibody

Antibody Characterization for Subcellular Localization

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HA.ll mouse monoclonal antibody (mAb) was obtained from Covance (USA), HA (3F10) rat mAb from Roche, SgII rabbit antibody from Meridian Life Science (USA), TGN38 mouse mAb from BD Biosciences (USA), tubulin mouse mAb from Developmental Hybridoma Bank Studies (USA), synaptotagmin 1 mouse antibody from Synaptic Systems (Germany), synaptophysin (p38) mAb from Covance (USA), HID-1 mouse mAb from Novus Biologicals (USA), and goat anti-rabbit Alexa Fluor 647, anti-mouse Alexa Fluor 488, anti-mouse Alexa Fluor 647, and anti-rat Alexa Fluor 488 secondary antibodies from Molecular Probes (USA). a2 rabbit polyclonal antibody was a generous gift from Xiao-Song Xie (UT Southwestern). The following antibodies did not work by Western blot: HID-1 mouse mAb from Novus Biologicals (2F4), HID-1 mouse mAb from LifeSpan BioSciences (clone 2E9), HID-1 goat polyclonal from Santa Cruz (S-14).

Hummer B.H., de Leeuw N.F., Burns C., Chen L., Joens M.S., Hosford B., Fitzpatrick J.A, & Asensio C.S. (2017). HID-1 controls formation of large dense core vesicles by influencing cargo sorting and trans-Golgi network acidification. Molecular Biology of the Cell, 28(26), 3870-3880.

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Comprehensive Immunofluorescence Labeling

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HA (3F10) rat mAb was obtained from Roche, SgII rabbit antibody from Meridian Life Science (USA), TGN38 mouse mAb from BD Biosciences (USA), tubulin and actin mouse mAb from Developmental Hybridoma Bank Studies (USA), HID-1 mouse mAb from Novus Biologicals (USA), GFP mouse mAb from Proteintech (USA), and goat anti-rabbit Alexa Fluor 647, anti-mouse Alexa Fluor 488, anti-mouse Alexa Fluor 647, and anti-rat Alexa Fluor 488 secondary antibodies from ThermoFisher (USA).

Hummer B.H., Carter T., Sellers B.L., Triplett J.D, & Asensio C.S. (2023). Identification of the functional domain of the dense core vesicle biogenesis factor HID-1. PLOS ONE, 18(9), e0291977.

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