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Busulfan bu

Manufactured by Merck Group
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Sourced in United States, Japan

Busulfan (BU) is a laboratory reagent used in various research and analytical applications. It is a bifunctional alkylating agent that can bind and cross-link DNA, disrupting cellular processes. Busulfan is commonly employed in research settings to study cell biology, DNA damage, and cellular responses, among other applications. The core function of Busulfan is to serve as a tool for researchers to investigate fundamental biological mechanisms and processes.

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Lab products found in correlation

Tacrolimus fk506, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions)

Close spelling variants of this product

Busulfan

3 protocols using busulfan bu

1

Induction of Aplastic Anemia in Rats

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Briefly, adult male Sprague-Dawley rats were given a 5-fluorouracil (5-FU) (J&K Scientific LLC, USA) injection (150 mg/kg) intraperitoneally in the first day. Then, busulfan (BU) (20 mg/kg) (Sigma-Aldrich, USA) was given intraperitoneally in the seventh day and was injected weekly. Fifty milligrams pure BU was dissolved in 5 mL acetone and was further diluted with 20 mL bacteriostatic water to make final concentration (2 mg/mL). The whole AA induction lasts 28 days. The values of red blood cell (RBC), white blood cell (WBC), and platelet (PLA) in peripheral blood should be decreased more than half at least. The evaluation of AA was mainly according to the examination of bone marrow, especially bone marrow biopsy [24 (link)–26 (link)].
Yang J., Zhang L., Wang H., Guo Z., Liu Y., Zhang Y., Wang C, & Li Q. (2017). Protective Effects of Chronic Intermittent Hypobaric Hypoxia Pretreatment against Aplastic Anemia through Improving the Adhesiveness and Stress of Mesenchymal Stem Cells in Rats. Stem Cells International, 2017, 5706193.
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2

Synthesis and Characterization of TM Mutants

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TM mutants TME5A (residues C387-C397), TME5B (residues C398-C408), and TME5C (residues C408-C426) were synthesized by the Peptide Institute Inc. (Osaka, Japan). The TME5C mutant with a single amino acid substitution was synthesized by GL Biochem (Shanghai, China). The amino acid sequences are listed in Table 1. Cyclophosphamide (CY) was purchased from Shionogi & Co., Ltd (Osaka, Japan). Busulfan (BU) and tacrolimus (FK506) were purchased from Sigma-Aldrich, Tokyo, Japan. TME5 and rTM were provided by Asahi Kasei Pharma (Tokyo, Japan).
Wang X., Pan B., Honda G., Wang X., Hashimoto Y., Ohkawara H., Xu K., Zeng L, & Ikezoe T. (2018). Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain. Haematologica, 103(10), 1730-1740.
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3

Conditioning and Transplantation of Engineered Bone Marrow

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Animals in experimental groups were conditioned with Busulfan (Bu, Sigma, St Louis, MO; injected intraperitoneally (i.p.) 25 mg/kg) at postnatal day8. Next day, fresh donor bone marrow cells from BoyJ mouse tibia, femur, and pelvis were isolate, counted and re-suspended into PBS. Each conditioned animal received 0.12 ml of the solution, containing about 5 × 107 total bone marrow cells via IP injection.
For transduced lineage negative bone marrow cells, total BoyJ bone marrow were isolated on the same day of mice conditioning. Isolated Lin− cells were transduced with lentiviral vectors (vTK1667 or vTK 1784) at MOI 50 (based on 293T cells) in its culture medium with additional 10ug/ml Rapamycin (Sigma, St Louis, MO) for 14~16hr. Transduced Lin− cells were washed with PBS and mixed with fresh isolated total bone marrow cells for transplantation. Each Bu conditioned mouse received 1 ×106 Lin− cells transduced with vTK1667, 1 ×106 Lin− cells transduced with vTK1784, and 1 ×107 fresh isolated total BM cells from BoyJ mice.
Hu P., Li Y., Nikolaishvili‐Feinberg N., Scesa G., Bi Y., Pan D., Moore D., Bongarzone E.R., Sands M.S., Miller R, & Kafri T. (2016). Hematopoietic Stem cell transplantation and lentiviral vector‐based gene therapy for Krabbe's disease: Present convictions and future prospects. Journal of Neuroscience Research, 94(11), 1152-1168.
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