Log In Sign up
The largest database of trusted experimental protocols

Negative sirnas universal control sicont

Manufactured by Merck Group
Visit Supplier

Negative siRNAs universal control (siCont) is a laboratory tool used in gene silencing experiments. It is a non-targeting small interfering RNA (siRNA) that serves as a negative control, allowing researchers to distinguish the effects of the experimental siRNA from non-specific effects.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (4 mentions) Predesigned sirnas for human fxr1, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

SiRNAs (112 citations) Control siRNA (99 citations) Universal negative siRNA (6 citations) Negative siRNA control (2 citations) SiCont (2 citations)

2 protocols using negative sirnas universal control sicont

1

Knockdown of FXR1 gene using siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Predesigned siRNAs for human FXR1 were purchased from Thermo Fisher Scientific Inc., Waltham, MA and negative siRNAs universal control (siCont) from Sigma-Aldrich. Reverse transfections were performed using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific Inc., Waltham, MA). At 48 h post-transfection, cells were harvested for further analysis. siRNA sequences are listed below:

FXR1 siRNA (siFXR1-1):

Sense: GGAAUGACUGAAUCUGAUAtt

Antisense: UAUCAGAUUCAGUCAUUCCat

FXR1 siRNA (siFXR1-2):

Sense: CGAGCUGAGUGAUUGGUCAtt

Antisense: UGACCAAUCACUCAGCUCGtc

George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand
2

Knockdown of FXR1 gene using siRNA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Predesigned siRNAs for human FXR1 were purchased from Thermo Fisher Scientific Inc., Waltham, MA and negative siRNAs universal control (siCont) from Sigma-Aldrich. Reverse transfections were performed using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific Inc., Waltham, MA). At 48 h post-transfection, cells were harvested for further analysis. siRNA sequences are listed below:

FXR1 siRNA (siFXR1-1):

Sense: GGAAUGACUGAAUCUGAUAtt

Antisense: UAUCAGAUUCAGUCAUUCCat

FXR1 siRNA (siFXR1-2):

Sense: CGAGCUGAGUGAUUGGUCAtt

Antisense: UGACCAAUCACUCAGCUCGtc

George J., Li Y., Kadamberi I.P., Parashar D., Tsaih S.W., Gupta P., Geethadevi A., Chen C., Ghosh C., Sun Y., Mittal S., Ramchandran R., Rui H., Lopez-Berestein G., Rodriguez-Aguayo C., Leone G., Rader J.S., Sood A.K., Dey M., Pradeep S, & Chaluvally-Raghavan P. (2021). RNA-binding protein FXR1 drives cMYC translation by recruiting eIF4F complex to the translation start site. Cell reports, 37(5), 109934.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!