Cell viability was tested by MTT assay. In brief, 2.0 × 103 HUVECs were sowed into 96 wells and allowed to adhere. After incubation for indicated times, 10 μL of 5 mg/mL MTT (Beyotime, Jiangsu, China) solution was added to each well of the 96 wells plate and allowed to incubate for additional 4 h. The supernatant was replaced by 150 μL of dimethyl sulfoxide (DMSO) and then shocked for 10 min. The absorbance was detected at 490 nm under a microplate spectrophotometer (Olympus, Tokyo, Japan).
Microplate spectrophotometer
Manufactured by Olympus
Sourced in Japan
The Olympus Microplate Spectrophotometer is a laboratory instrument used for the quantitative analysis of samples in microplate format. It measures the absorbance or optical density of samples in a microplate, allowing for the determination of various parameters such as concentration, enzyme activity, and cell growth.
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2 protocols using microplate spectrophotometer
1
Quantifying HUVEC Viability via MTT Assay
2
Quantifying Inflammatory Cytokine Levels
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The concentrations of interleukin 6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α) in medium were evaluated by IL-6 ELISA KIT, IL-1β ELISA KIT, and TNF-α ELISA KIT (Boster, Wuhan, China) as instructed by the manufacturer, respectively. The absorbance was measured under a microplate spectrophotometer (Olympus).
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