Anti mbp

Manufactured by GeneTex

Anti-MBP is a laboratory product that can be used to detect the presence of myelin basic protein (MBP) in samples. MBP is a structural protein found in the myelin sheath of nerve cells. The Anti-MBP product provides a tool for researchers to investigate the role of MBP in various biological processes.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Anti lamp2, by Abcam (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Abcam (1 mentions) Donkey anti rabbit igg alexa fluor 647, by Abcam (1 mentions) Fv3000, by Olympus (1 mentions) Chaps, by Thermo Fisher Scientific (1 mentions) Chaps, by Merck Group (1 mentions) Anti atm, by Santa Cruz Biotechnology (1 mentions) Nupage 4 12 bis tris, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by New England Biolabs (1 mentions) Dynabeads m 280 streptavidin, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti mbp

Multimodal Immunolabelling of Neural Cells

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Cells were fixed with 4% PFA in PBS for 15 min, then blocked and permeabilized with 0.3% Triton-X in bovine serum albumin (BSA, 3%) for 15 min. For LAMP-2 immunolabelling, cells were permeabilized with 0.01% saponin as per manufacturer’s instructions, followed by blocking with 3% BSA for 30 min. Cells were incubated with the following primary antibodies overnight at 4 °C; anti-p75ntr (4 µg/ml; BioLegend); anti-S100β (4 µg/ml; ThermoFisher) and anti-LAMP-2 (4.2 µg/ml; Abcam), anti-MBP (4.3 µg/ml; Gene Tex) The following day, cells were incubated with secondary antibodies donkey anti-rabbit IgG Alexa Fluor 488 (4 µg/ml; Abcam), donkey anti-rat IgG Alexa Fluor 647 (4 µg/ml; Abcam) or donkey anti-rabbit IgG Alexa Fluor 647 (4 µg/ml; Abcam; ThermoFisher). Nuclei were stained using Hoechst 33,342 (1 μg/ml, ThermoFisher). High resolution images were obtained using a confocal microscope (Olympus FV3000).

Nazareth L., Shelper T.B., Chacko A., Basu S., Delbaz A., Lee J.Y., Chen M., St John J.A, & Ekberg J.A. (2020). Key differences between olfactory ensheathing cells and Schwann cells regarding phagocytosis of necrotic cells: implications for transplantation therapies. Scientific Reports, 10, 18936.

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ATM Binding to MRI Domains

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40 nM recombinant biotin-FLAG-tagged human ATM was incubated with MBP-tagged human MRI, MRI^ΔN, or MRI^ΔC (50, 100, or 200 nM) and 100 ng bovine serum albumin (BSA, New England Biolabs) in A buffer (25 mM Tris-HCl pH 8.0, 100 mM NaCl, 10% v/v glycerol) at room temperature for 15 minutes in a final volume of 30 μL. 2 μL of Dynabeads M-280 Streptavidin (Thermo Fisher) were pre-washed with 1 mg/mL BSA and 1% CHAPS (Sigma). The samples were then added to the bead mixture with 0.1% CHAPS in A buffer and incubated on ice for 15 minutes. After three washes with 2 μg/mL BSA in A buffer with 0.1% CHAPS, proteins bound to the beads were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris (Thermo Fisher), followed by western blotting with anti-ATM (Santa Cruz) or anti-MBP (GeneTex).

Hung P.J., Johnson B., Chen B.R., Byrum A.K., Bredemeyer A.L., Yewdell W.T., Johnson T.E., Lee B.J., Deivasigamani S., Hindi I., Amatya P., Gross M.L., Paull T.T., Pisapia D.J., Chaudhuri J., Petrini J.J., Mosammaparast N., Amarasinghe G.K., Zha S., Tyler J.K, & Sleckman B.P. (2018). MRI is a DNA Damage Response Adaptor during Classical Non-Homologous End Joining. Molecular cell, 71(2), 332-342.e8.

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