Agonistic InVivoMAb aOX40 antibody (CD134, clone OX-86) or InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase (clone HRPN) was obtained from BioXCell (West Lebanon, NH, USA). For the depletion experiments, 100 μg of anti-CD4 antibody (clone GK1.5, BioXCell, West Lebanon, LH, USA) or anti-CD8 antibody (clone 2.43, BioXCell) or InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin (clone LTF-2, BioXCell) was administered one week before tumor inoculation and then once weekly to achieve depletion of CD4+ or CD8+ T cells. Successful eradication was confirmed by flow cytometry.
Anti keyhole limpet hemocyanin clone ltf 2
Manufactured by BioXCell
Sourced in United States
Anti-keyhole limpet hemocyanin (clone LTF-2) is a laboratory reagent used as a carrier protein in immunological assays and experiments. It is a protein derived from the keyhole limpet, Megathura crenulata.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti keyhole limpet hemocyanin clone ltf 2
1
CD4+ and CD8+ T-Cell Depletion Protocol
2
Evaluating Immune Checkpoint Inhibitor Efficacy in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All in vivo experiments were treated with intraperitoneal injections. Mice were treated twice a week with 200 ug of InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin (clone LTF-2, BioXcell BE0090), InVivoMab anti-mouse CTLA-4 (clone 9H10, BioXcell BE 0131), and InVivoMab anti-mouse PDL-1 (clone 10F9G2, BioXcell BE0101). Mice were treated until tumors reached 2 cm in one direction. Mouse weight was monitored and recorded weekly. Tumor volumes were measured and plotted as mean total tumor burden ± SEM. Significant differences in tumor fold change were measured by a two-way analysis of variance (ANOVA) multiple comparisons on ranks. The statistical significance of survival curves was assessed using the Kaplan-Meier log-rank analysis. All statistical analysis was performed in Prism version 7 (Graphpad, Inc.). P values are designated as *P < 0.05, **P < 0.01, and ***P < 0.001.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!