Hplc grade methanol

Imidazol formate, formic acid, acetonitrile, sulfatase, 2-propanol, 2,2-azinobis-(ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,2-benzenedithiol, 3,3′-diindolylmethane (DIM), and indole-3-carbinol (I3C) were obtained from Sigma-Aldrich (Darmstadt, Germany). Glucotropaeolin (GTL) was from AppliChem (Darmstadt, Germany), indole-3-acetonitrile (I3ACN) from Merck (Darmstadt, Germany), and HPLC grade methanol from Chempur (Piekary Śląskie, Poland). Water was purified with a QPLUS185 system from Millipore (Burlington, MA, USA).

HPLC grade acetone, ethyl acetate, and meta-phosphoric acid were obtained from Sigma-Aldrich (Poznań, Poland). HPLC grade acetonitrile, HPLC grade methanol, ortho-phosphoric acid, and ultrapure magnesium carbonate were obtained from Chempur (Piekary Śląskie, Poland). Standards of β-carotene, caffeic acid, chlorogenic acid, chlorophyll a, chlorophyll b, dehydroascorbic acid, ferulic acid, gallic acid, kaempferol-3-O-glucoside, lutein, L-ascorbic acid, p-coumaric acid, and quercetin-3-O-rutinoside (HPLC grade, 99.5–99.9% pure) were obtained from Fluka and Sigma-Aldrich (Poznań, Poland). Standards of fructose (99.9% CAS 57-48-7), glucose (99.9% CAS 50-99-7), and sucrose (99.9% CAS 57-50-1) were obtained from Sigma-Aldrich (Warsaw, Poland).