DMEM, fetal bovine serum (FBS) (Gibco/invitrogen, Carlsbad, CA, USA), a Cell Counting Kit-8 assay (Dojindo Lab, Kumamoto, Japan), β-glucan kit (Megazyme International, Wicklow, Ireland), Phagocytosis Assay Kit (Cayman, MI, USA), cyclophosphamide (Sigma Aldrich, St.Louis, MO, USA) β-1,3-glucan (Sigma Aldrich, St.Louis, MO, USA) and cordycepin (Sigma Aldrich, St.Louis, MO, USA) were purchased. Phospho-Lyn antibody, Lyn antibody, phospho-Syk antibody, Syk antibody, phospho-ERK antibody, ERK antibody, phospho-p38 antibody, p38 antibody, phospho-JNK antibody, JNK antibody, NFκB antibody, phospho-IκB antibody and IκB antibody were obtained from Cell signaling Technology Inc. (Danvers, MA, USA). β-Actin antibody was obtained from Santa Cruz (Dallas, TX, USA).
Syk antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Syk antibody is a laboratory reagent used for the detection of the Syk protein in various experimental applications. Syk is a tyrosine kinase that plays a crucial role in signal transduction pathways. The antibody can be used to study the expression and localization of Syk in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Nf κb antibody, by Cell Signaling Technology (1 mentions)
Erk antibody, by Cell Signaling Technology (1 mentions)
Phospho erk antibody, by Cell Signaling Technology (1 mentions)
Jnk antibody, by Cell Signaling Technology (1 mentions)
Phospho jnk antibody, by Cell Signaling Technology (1 mentions)
Cyclophosphamide, by Merck Group (1 mentions)
Iκb antibody, by Cell Signaling Technology (1 mentions)
Cordycepin, by Merck Group (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
P38 antibody, by Cell Signaling Technology (1 mentions)
Phagocytosis assay kit, by Cayman Chemical (1 mentions)
β 1 3 glucan, by Merck Group (1 mentions)
Phospho p38 antibody, by Cell Signaling Technology (1 mentions)
Tritc conjugated phalloidin, by Merck Group (1 mentions)
Alexa fluor 555 labeled donkey anti rabbit igg, by Beyotime (1 mentions)
Fitc labeled goat anti rabbit igg, by Beyotime (1 mentions)
2 protocols using syk antibody
1
Immunomodulatory Effects of Beta-Glucans
2
Immunostaining of SYK and Actin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunostaining was performed followed with our previously described method (Liu et al. 2013 (link)) but not the permeabilization step. The antibodies included SYK antibody (1:400, Cell-Signaling Technology, Danvers, USA), actin antibody (1:400, Sigma, St. Louis, USA), FITC-labeled goat anti-rabbit IgG, Alexa Fluor 555-labeled donkey anti-rabbit IgG, Alexa Fluor 350-labeled goat anti-mouse IgG (1:400, Beyotime Institute of Biotechnology, China), and TRITC-conjugated Phalloidin (1:1000, Sigma, St. Louis, USA).
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