Intellifish hybridization buffer

Manufactured by Abbott

Sourced in United States

The IntelliFISH Hybridization buffer is a laboratory reagent designed to facilitate the hybridization process during fluorescence in situ hybridization (FISH) experiments. It is a buffered solution that helps maintain the optimal conditions for the binding of DNA or RNA probes to their target sequences within cells or tissue samples.

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Lab products found in correlation

Vectashield hardset, by Vector Laboratories (1 mentions) Spectrum green, by Abbott (1 mentions) Spectrum orange, by Abbott (1 mentions) Citrisolv hybrid, by Decon Labs (1 mentions) Vectashield containing dapi, by Vector Laboratories (1 mentions)

2 protocols using intellifish hybridization buffer

FISH Protocol for FFPE Samples

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A standard protocol was established for FISH on formalin fixed, paraffin embedded (FFPE) specimens. A tissue-micro-array (TMA) with ten cores (at 3mm² diameter) for the probe set up and 42 diagnostic samples including core needle and excisional biopsies were analyzed. Adapted to the tissue type, the pretreatment time varied from 30 to 40 min. The specific probes (Zytovision, Germany or Vysis, Abbott Molecular, USA) were hybridized at 37 °C for 4 h in the presence of the IntelliFISH Hybridization buffer (Vysis, Abbott Molecular, USA). As nuclear stain and mounting medium the DAPI (4′,6 diamidino-2-phenylindole) VECTASHIELD® HardSet™ (Vector laboratories, CA, USA) with a minimum hardening time of 30 min was used.

Chea V., Pleiner V., Schweizer V., Herzog B., Bode B, & Tinguely M. (2021). Optimized workflow for digitalized FISH analysis in pathology. Diagnostic Pathology, 16, 42.

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CDKN2A Gene Aberration Evaluation

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CDKN2A FISH was performed only in the two cases with negative p16 IHC. FISH was performed on interphase nuclei using a DNA probe in the short arm of chromosome 9 (LSI CDKN2A localizing to 9p21) labeled in Spectrum Orange simultaneously with a probe for the centromere of chromosome 9 as a control (CEP9 localizing to 9p11-q11) labeled in Spectrum Green (Abbott Molecular, Des Plains, IL). Slides containing FFPE tissue were deparaffinized in Citrisolv Hybrid (Decon Labs, King of Prussia, IL) followed by sequential treatment with 0.2N HCl, 1M sodium thiocyanate, Protease I (Abbott Molecular), 10% formalin, and dehydrating ethanol series (70%, 85%, and 95%). Tissue and probe were codenatured by heating at 80°C for 2 minutes using a ThermoBrite instrument (Abbott Molecular). Hybridization was performed overnight at 37°C in IntelliFISH hybridization buffer (Abbott Molecular). Finally, the slides were mounted with Vectashield containing DAPI (Vector Laboratories, Burlingame, CA). After reviewing the corresponding H&E stained slide with a pathologist the tissue was analyzed by 2 screeners for 200 interphase nuclei per region of interest.

Matson D.R., Accola M.A., Henderson L., Shao X., Frater-Rubsam L., Horner V.L., Rehrauer W.M., Weisman P, & Xu J. (2021). A “null” pattern of p16 immunostaining in endometrial serous carcinoma: An under-recognized and important aberrant staining pattern. International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, 41(4), 378-388.

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