HFF cells (1x105) were infected at an MOI of 1 for 72 h. DNA was extracted from infected cells and sheared using a Covaris S220 to an approximate size of 450 bp. Sequencing libraries were created using a Kapa LTP Library preparation kit according to the manufacturer’s instructions, employing indexed primers (New England Biolabs) for PCR. The libraries were sequenced on a NextSeq Mid Output 300 cycle cartridge to produce approximately 5 million paired-end reads of 150 nucleotides (nt). Sequence data have been deposited in the European Nucleotide Archive (ENA) at EMBL-EBI under accession number PRJEB58764.
Ltp library preparation kit
Manufactured by New England Biolabs
The LTP Library preparation kit is a product offered by New England Biolabs, designed for the construction of DNA libraries for next-generation sequencing. The kit provides the necessary reagents and protocols to prepare DNA samples for sequencing on various platforms.
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Lab products found in correlation
2 protocols using ltp library preparation kit
1
RNA-Seq analysis of HFF cell infection
2
HFF Cell Infection and Sequencing
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HFF cells (1 × 105) were infected at an MOI of 1 for 72 h. DNA was extracted from infected cells and sheared using a Covaris S220 to an approximate size of 450 bp. Sequencing libraries were created using a Kapa LTP Library preparation kit according to the manufacturer’s instructions, employing indexed primers (New England Biolabs) for PCR. The libraries were sequenced on a NextSeq Mid Output 300 cycle cartridge to produce approximately 5 million paired-end reads of 150 nucleotides (nt).
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