Mouse anti β actin monoclonal antibody mab

The chicken fibroblast cell line (Douglas Foster 1, DF-1) and human embryo kidney cell line (293T) were maintained in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS). The DF-1 and 293T cells were cultured at 37 °C in a cell incubator under 5% CO₂. The cell lines were regularly tested in-house for mycoplasma infection. RIPA buffer, streptomycin, penicillin, mouse anti-β-actin monoclonal antibody (mAb), horseradish-peroxidase-conjugated goat anti-mouse IgG antibody, and hemagglutinin (HA)-tagged and FLAG-tagged mAbs were purchased from Sigma-Aldrich (St. Louis, MO, USA). Recombinant Chicken Interferon Alpha was purchased from Bio-Rad (Hercules, CA, USA). Rabbit anti-phospho-STAT1 (p-STAT; S727), anti-STAT1 (ab92506), anti-Mertk (ab300136) were purchased from Abcam (Cambridge, UK). SDS-PAGE sample loading buffer and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Beyotime (Nantong, China). Mut Express® II Fast Mutagenesis Kit V2 was purchased from Vazyme Biotect Co., Ltd. (Nanjing, China) for site-directed mutagenesis. TRIzol Reagent, FBS, DMEM, enhanced chemiluminescent reagent (ECL), Alexa-Fluor®−594- and Alexa-Fluor®−488-conjugated goat anti-mouse secondary antibodies were obtained from Thermo Fisher Scientific (Carlsbad, CA, USA). The mAb directed against the NDV nucleoprotein (NP) was prepared in our laboratory.