Dulbecco s modified eagle media dmem growth media

Poly(ethylene glycol) methyl ether-grafted disulfide-poly(β-amino esters) (PAE3100-ss-mPEG2000) and poly(ethylene glycol) methyl ether-b-poly(β-amino esters) (mPEG5000-b-PAE4090) were synthesized as reported in our previous works [15 (link),31 (link)]. Doxorubicin hydrochloride (DOX-HCl) was purchased from Wuhan Yuan Cheng Gong Chuang Co. Ltd. (Wuhan, China). Triethylamine (TEA, >99%), pyrene (99%), DL-dithiothreitol (DTT, which was used to replace GSH in this study), dichloromethane (DCM), dimethyl sulfoxide (DMSO), chloroform, and all other chemical reagents were used as received. Methylthiazoltetrazolium (MTT) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified eagle media (DMEM) growth media, fetal bovine serum (FBS), trypsin, penicillin, and streptomycin were all purchased from Invitrogen (Carlsbad, NM, USA); HepG2 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, MA, USA) and all other reagents were used as received.

Doxorubicin hydrochloride (DOX-HCl) was purchased from Wuhan Yuan Cheng Gong Chuang Co. Bovine serum albumin (BSA, ≥98%), glutaric acid (≥99%), glutaraldehyde, triethylamine (TEA, ≥99%) and methylthiazoltetrazolium (MTT) were purchased from Sigma (St. Louis, MO). Dulbecco’s modified eagle media (DMEM) growth media, fetal bovine serum (FBS), trypsin, penicillin and streptomycin, were all purchased from Invitrogen; NIH 3T3, A549, A2780 and NCL-H460 cell lines were obtained from the American Type Culture Collection (ATCC). All other reagents were used as received.