Lps l2630 10mg

LPS (L2630-10MG) was purchased from Sigma-Aldrich (USA). The cytokines were measured in the testis and serum using ELISA kits. The kits were obtained from ELK Biotechnology (Wuhan, China). The levels of the cytokines interleukin 1alpha (IL-1α, Cat num: ELK1148), interleukin 1beta (IL-1β, Cat num: ELK1272), tumor necrosis factor-alpha (TNF-α, Cat num: ELK1396), monocyte chemotactic protein 1 (MCP-1, Cat num: ELK5504) and interleukin 6 (IL-6, Cat num: ELK1158) were assessed. Oxidative stress-related indicators assay kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Glutathione peroxidase (GSH-PX, Cat num: A005-1) and total antioxidant capacity (TAOC, Cat num: A015-1) were measured using colorimetry.11 (link),12 (link) Catalase (CAT, Cat num: A007-1), superoxide dismutase (SOD, Cat num: A001-1) and malondialdehyde (MDA, Cat num: A003-1) were measured by spectrophotometry.13 (link),14 (link)

LPS (L2630-10MG) was purchased from Sigma-Aldrich, Co., (USA). LYC was obtained from the Nanjing Yuanjian Biotechnology Co., LTD (Nanjing, China).
After two weeks of acclimatization, the rats were split into four groups. The first was the blank control group, and the second was given oil (5 mL/kg/d, 4 weeks) as the vehicle control group. The third group received LPS (5 mg/kg), dissolved in 0.9% sodium chloride injection, intravenously. The epididymal inflammatory response was induced by intraperitoneal injection of LPS in SD rats. Intragastric administration of LYC was started 24 h after the LPS injection. LPS (5 mg/kg, dissolved in 0.9% sodium chloride injection, i.p.) and LYC (5 mg/kg/d, dissolved in oil, i.g., 4 weeks) were administered to the fourth group. This study was approved by the Experimental Animal Ethics Committee of East Region Military Command General Hospital (Jinling Hospital, 2022JLHSXJDWLS-0021) and carried out according to the National Institutes of Health protocol to laboratory animal care and use.