After the exposure, the same sagittal sections used for autoradiography were fixed in cold acetone (approx. -15 °C), quickly dried under a fan and blocked with 20% normal goat serum for 1 hour at RT. After disposing the goat serum, the tissues were incubated with a Goat anti-human IgG [H+L] Cross-Adsorbed Secondary HRP-Antibody (Invitrogen; 0.4 µg/mL, 1:2000) at RT for 1 hour under dark conditions. After disposing of the antibody solution, the tissue was washed three times with 0.05% Tween-20 in PBS for 5 minutes each, followed by a final wash step with deionized water (dH2O) for 5 minutes. Subsequently, the tissue was incubated with a 0.125% freshly filtered Thioflavin S solution at RT for 8 minutes under dark conditions. After disposing the Thioflavin S solution, the tissue was washed for 3 minutes each in the following order; 2x80% EtOH, 1x90% EtOH, 3xdH2O. The tissues were mounted with ProLong™ Gold Antifade Mountant (Invitrogen™, P36930). Images of the stained sections were taken with a fluorescence microscope (Zeiss Axio Observer with a Colibri 7 LED light source and an Axiocam 506 monochrome camera) and equally processed using the Zen blue software Version 3.4.
Goat anti human igg h l cross adsorbed secondary hrp antibody
Manufactured by Thermo Fisher Scientific
Goat anti-human IgG [H+L] Cross-Adsorbed Secondary HRP-Antibody is a laboratory reagent used as a detection tool in immunoassays. It is a secondary antibody that binds to human immunoglobulin G (IgG) antibodies, with specificity for both the heavy (H) and light (L) chains. The antibody is conjugated with horseradish peroxidase (HRP), an enzyme that can be used for colorimetric or chemiluminescent detection.
Automatically generated - may contain errors
2 protocols using goat anti human igg h l cross adsorbed secondary hrp antibody
1
Immunohistochemical Staining of IgG and Amyloid
2
Immunohistochemical Staining Protocol for Amyloid Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the exposure, the same sagittal sections used for autoradiography were fixed in cold acetone (approx. -15 °C), quickly dried under a fan, and blocked with 20% normal goat serum for 1 h at RT. The tissues were then incubated with a Goat anti-human IgG [H + L] Cross-Adsorbed Secondary HRP-Antibody (Invitrogen; 0.4 µg/mL, 1:2000) at RT for 1 h under dark conditions. The tissues were washed 3 × 5 min with 0.05% Tween-20 in PBS, followed by a final wash step with deionized water (dH 2 O) for 5 min. Subsequently, the tissues were incubated with a 0.125% freshly filtered Thioflavin S solution at RT for 8 min under dark conditions. Then, the tissues were washed for 3 min in each of the following solutions: 2 × 80% EtOH, 1 × 90% EtOH, 3 × dH 2 O. The tissues were mounted with ProLong™ Gold Antifade Mountant (Invitrogen™, P36930). Images of the stained sections were taken with a fluorescence microscope (Zeiss Axio Observer with a Colibri 7 LED light source and an Axiocam 506 monochrome camera) and equally processed using the Zen blue software Version 3.4.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!