FL cells were thawed and allowed to recover at 37 °C for an hour prior to staining. Cells were blocked with TruStain FcX (BioLegend, 422301) and stained with TotalSeq A antibody mix containing 1ug of each TotalSeq A antibody per condition (BioLegend, see Table S1 for a list of antibodies). Anti-human CD235a-APC antibody (BD Biosciences, 551336) was added to the CD34− fraction. The CD34+ fraction was stained with anti-human CD34-APC antibody (BD Biosciences, 555824) and anti-human GPI-80-PE antibody (MBL International, D087-5). All samples were stained with calcein blue (Invitrogen, C34853) for live/dead exclusion and cell populations were sorted (Beckman Coulter MoFlo Astrios) as shown in Fig. S1A prior to loading onto the 10x Genomics platform. Chromium Single-Cell 3ʹ Reagent Kit v3 with Feature Barcoding technology for Cell-Surface Protein was used and the recommendations from the manufacturer were followed as specified in 10x Genomics user guide document number CG000185, Rev B.
Anti human cd34 apc antibody
Manufactured by BD
The Anti-human CD34-APC antibody is a fluorescently-labeled monoclonal antibody that binds to the CD34 cell surface antigen found on hematopoietic stem and progenitor cells. It is commonly used in flow cytometry applications for the detection and analysis of CD34-positive cell populations.
Automatically generated - may contain errors
2 protocols using anti human cd34 apc antibody
1
Single-Cell Profiling of Hematopoietic Cells
2
Single-Cell RNA-Seq Profiling of Hematopoietic Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FL cells were thawed and allowed to recover at 37°C for an hour prior to staining.
Cells were blocked with TruStain FcX (BioLegend, 422301) and stained with TotalSeq A antibody mix containing 1ug of each TotalSeq A antibody per sample (BioLegend, see Table S1 for a list of antibodies). Anti-human CD235a-APC antibody (BD Biosciences, 551336) was added to the CD34 -sample. The CD34 + fraction was stained with anti-human CD34-APC antibody (BD Biosciences, 555824) and anti-human GPI-80-PE antibody (MBL International, D087-5). All samples were stained with calcein blue (Invitrogen, C34853) for live/dead exclusion and cell populations were sorted as shown in Figure S1A prior to loading onto the 10X Genomics platform. Chromium Single Cell 3ʹ Reagent Kit v3 with Feature Barcoding technology for Cell Surface Protein was used and the recommendations from the manufacturer were followed as specified in 10x
Genomics user guide document number CG000185, Rev B.
Cells were blocked with TruStain FcX (BioLegend, 422301) and stained with TotalSeq A antibody mix containing 1ug of each TotalSeq A antibody per sample (BioLegend, see Table S1 for a list of antibodies). Anti-human CD235a-APC antibody (BD Biosciences, 551336) was added to the CD34 -sample. The CD34 + fraction was stained with anti-human CD34-APC antibody (BD Biosciences, 555824) and anti-human GPI-80-PE antibody (MBL International, D087-5). All samples were stained with calcein blue (Invitrogen, C34853) for live/dead exclusion and cell populations were sorted as shown in Figure S1A prior to loading onto the 10X Genomics platform. Chromium Single Cell 3ʹ Reagent Kit v3 with Feature Barcoding technology for Cell Surface Protein was used and the recommendations from the manufacturer were followed as specified in 10x
Genomics user guide document number CG000185, Rev B.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!