William s medium e wme

Manufactured by Thermo Fisher Scientific

Sourced in United States

William's Medium E (WME) is a cell culture medium designed to support the growth and maintenance of various cell types, including hepatocytes and other liver-derived cells. It provides a balanced formulation of nutrients, vitamins, and other components necessary for optimal cell growth and function.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using william s medium e wme

Rhodamine 123 Permeability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rhodamine 123 (RHD123), dimethyl sulfoxide (DMSO), acetonitrile (ACN), ethanol, low-gelling temperature agarose (type VII-A), amphotericin B solution (250 μg/ml), D-glucose, HEPES, RIF, sodium chloride, ethylenediaminetetraacetic acid (EDTA), formic acid, Tris-HCl, dichloromethane, sodium acetate, CP100356 [a model ABCB1 inhibitor (Martinec et al., 2019 (link))], and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). All chemicals were of at least analytical grade. William’s Medium E (WME) with Glutamax-I and gentamicin (50 μg/ml) solution was obtained from Thermo Fisher (Waltham, MA, United States). Formaldehyde solution (4%) was purchased from Mallinckrodt Baker B.V. (Deventer, Netherlands). Stock solutions of RIF and CP100356 used in all experiments were prepared in DMSO.

Martinec O., Biel C., de Graaf I.A., Huliciak M., de Jong K.P., Staud F., Cecka F., Olinga P., Vokral I, & Cerveny L. (2021). Rifampicin Induces Gene, Protein, and Activity of P-Glycoprotein (ABCB1) in Human Precision-Cut Intestinal Slices. Frontiers in Pharmacology, 12, 684156.

+ Open protocol

+ Expand

Isolation and Characterization of Mitochondria

Check if the same lab product or an alternative is used in the 5 most similar protocols

LPS from Escherichia coli O127:B8 (lot number: 0000082455, 1.5 × 10 6 endotoxin units/mg), perchloric acid, thiobarbituric acid, antimycin A, decylubiquinone, rotenone, dichloroindophenol, oligomycin, and rhodamine 123 were purchased from Millipore-Sigma (St. Louis, MO, USA). Cytochrome c, 1,1,3,3-tetraethoxypropane, cyclosporin A, collagenase, dexamethasone, and insulin were purchased from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan). ADP, ATP, pyruvate kinase, and lactate dehydrogenase were purchased from Oriental Yeast (Tokyo, Japan). Carboxyatractyloside and bongkrekic acid were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). William's medium E (WME) and GlutaMAX TM were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum (FBS) was purchased from Biosera (Nuaille, France). Antibiotic-antimycotic solutions were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Type I collagen (rat tail) was purchased from Corning (Bedford, MA, USA).

Nakajima R., Takemura A., Ikeyama Y, & Ito K. (2023). Lipopolysaccharide administration increases the susceptibility of mitochondrial permeability transition pore opening via altering adenine nucleotide translocase conformation in the mouse liver. The Journal of toxicological sciences, 48(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!