For two-photon excitation microscopy (2PM), we used an FV1200MPE-IX83 inverted microscope (Olympus) equipped with a 30×/1.05 NA silicon oil-immersion objective lens (UPLSAPO 30XS; Olympus), an LCV110-MPE incubator microscope (Olympus) equipped with a 25×/1.05 water-immersion objective lens (XLPLN 25XWMP2; Olympus), and an InSight DeepSee Laser (Spectra Physics). The laser power was set to 3–18%. The scan speed was set between 4–12.5 μs per pixel. Z-stack images were acquired at 1–10 μm intervals. In time-lapse analyses, images were recorded every 1–3 min. The excitation wavelength for CFP was 840 nm. We used an IR-cut filter (BA685RIF-3), two dichroic mirrors (DM505 and DM570), and two emission filters (BA460-500 for CFP and BA520-560 for YFP) (Olympus). Confocal images were acquired with an FV1000/IX83 confocal microscope (Olympus) equipped with a 30×/1.05 NA silicon oil-immersion objective lens (UPLSAPO 30XS; Olympus).
Ba685rif 3
Manufactured by Olympus
The BA685RIF-3 is a compact and versatile laboratory microscope designed for a range of applications. It features binocular observation, coaxial coarse and fine focusing controls, and LED illumination. The microscope is suitable for various educational and research tasks that require high-quality microscopic observation.
Automatically generated - may contain errors
Lab products found in correlation
Ba460 500, by Olympus (3 mentions)
Ba520 560, by Olympus (3 mentions)
Dm505, by Olympus (3 mentions)
Dm570, by Olympus (2 mentions)
Uplsapo30xs, by Olympus (2 mentions)
Fv1000 ix83 confocal microscope, by Olympus (2 mentions)
Fv1200mpe ix83 inverted microscope, by Olympus (2 mentions)
Insight deepsee laser, by Spectra-Physics (2 mentions)
Xlpln25xwmp, by Olympus (2 mentions)
Lcv110 mpe incubator microscope, by Olympus (1 mentions)
Xlpln25xwmp2, by Olympus (1 mentions)
Fv1200mpe bx61wi, by Olympus (1 mentions)
Metamorph software, by Universal Imaging (1 mentions)
Rdm690, by Olympus (1 mentions)
Fv1000mpe bx61wi upright microscope, by Olympus (1 mentions)
Dm505, by IDEX Corporation (1 mentions)
Dm570, by IDEX Corporation (1 mentions)
Insight deepsee ultrafast laser, by Spectra-Physics (1 mentions)
Ff01 425 30, by IDEX Corporation (1 mentions)
Ff01 472 30, by IDEX Corporation (1 mentions)
3 protocols using ba685rif 3
1
Two-photon Excitation Microscopy for Live-cell Imaging
2
Two-Photon Excitation Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For two-photon excitation microscopy (2PM), we used an FV1200MPE-IX83 inverted microscope (Olympus, Tokyo, Japan) equipped with a 30x/1.05 NA silicon oil-immersion objective lens (UPLSAPO 30XS; Olympus) and an FV1200MPE-BX61WI upright microscope equipped with a 25x/1.05 water-immersion objective lens (XLPLN 25XWMP; Olympus) and an InSight DeepSee Laser (Spectra Physics, Santa Clara, CA, USA). The laser power was set to 8–10% and 2–4% for the observation of the intestine and the skin, respectively [33 (link), 38 (link)]. The scan speed was set at 20 μs/pixel. We used 840-nm light to excite CFP. We used an IR-cut filter (BA685RIF-3), two dichroic mirrors (DM505 and DM570), and two emission filters (BA460-500 for CFP and BA520-560 for YFP) (Olympus). Acquired images were analyzed with MetaMorph software (Universal Imaging, West Chester, PA, USA) as described previously [34 (link), 39 (link)].
Confocal images were acquired with an FV1000/IX83 confocal microscope (Olympus) equipped with a 30x/1.05 NA silicon oil-immersion objective lens (UPLSAPO 30XS; Olympus).
Confocal images were acquired with an FV1000/IX83 confocal microscope (Olympus) equipped with a 30x/1.05 NA silicon oil-immersion objective lens (UPLSAPO 30XS; Olympus).
3
Multimodal Imaging with Upright Microscope
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used an FV1000MPE-BX61WI upright microscope (Olympus, Tokyo, Japan) equipped with a 25Â/1.05 waterimmersion objective lens (XLPLN 25XWMP; Olympus) and an InSight DeepSee Ultrafast laser (0.95 W at 900 nm; Spectra Physics, Mountain View, CA). The excitation wavelength for cyan fluorescent protein (CFP) was 840 nm. An infrared lightecut filter, BA685RIF-3 (Olympus); two dichroic mirrors, DM505 and DM570 (Olympus); and four emission filters, FF01-425/30 (Semrock, Rochester, NY) for the second harmonic generation, BA460-500 (Olympus) for CFP, BA520-560 (Olympus) for yellow fluorescent protein, and 645/60 (Chroma Technology, Bellows Falls, VT) for Qtracker 655 (Life Technologies, Carlsbad, CA), were used. Qtracker 655 is intravenously administered with other reagents to confirm drug delivery to target organs. For Fucci mouse imaging, an IR-cut filter, RDM690 (Olympus); two dichroic mirrors, DM505 and DM570; and three emission filters, FF01-472/30 (Semrock) for second harmonic generation images, BA495-540 (Olympus) for mAG, and BA575-630 (Olympus) for mKO2, were used. The microscope was equipped with a two-channel GaAsP detector unit and two built-in photomultiplier tubes. FluoView software version 4.1a (Olympus) was used to control the microscope and to acquire images, which were saved in the multilayer 16-bit tagged image file format.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!