The following antibodies were used in the experiments: allophycocyanin (APC)-conjugated mouse anti-CD44 from BD Biosciences, phycoerythrin (PE)-conjugated mouse anti-CD133 from Miltenyi Biotec, rabbit anti-CD44, rabbit anti-CD133, and mouse anti-OGT from Abcam, Alexa 647-conjugated rabbit anti-mouse from Invitrogen; rabbit anti-β-tubulin from Cell Signaling Technology (Danvers, MA, USA); Alexa 488-conjugated goat anti-rabbit from Molecular Probes, Inc., (Eugene, OR, USA), mouse anti-O-GlcNAc (RL2) from Thermo Fisher Scientific; mouse anti-GAPDH from Santa Cruz Biotechnoloigy Inc (Sta. Cruz, CA, USA).
Pe conjugated mouse anti cd133
Manufactured by Miltenyi Biotec
Sourced in United States
The PE)-conjugated mouse anti-CD133 is a laboratory reagent used to detect and analyze CD133-expressing cells. CD133 is a cell surface marker commonly used to identify stem and progenitor cells. The PE (phycoerythrin) fluorescent label allows for the detection and quantification of CD133-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Alexa 488 conjugated goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Apc conjugated mouse anti cd44, by BD (1 mentions)
Mouse anti o glcnac rl2, by Thermo Fisher Scientific (1 mentions)
Rabbit anti β tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti cd44, by Abcam (1 mentions)
Rabbit anti cd133, by Abcam (1 mentions)
2 protocols using pe conjugated mouse anti cd133
1
Investigating Cancer Stem Cell Markers
2
Identification of LGR5 and CD133/CD44 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were dissociated and washed twice in PBS. Subsequently, cell suspensions were incubated with 1:50 PE-conjugated mouse anti-LGR5 Ab (OriGene, Rockville, MD, USA), or 1:10 PE-conjugated mouse anti-CD133 (Miltenyi Biotec, Bergisch Gladbach, Germany) and 1:10 FITC-conjugated mouse anti-CD44 (BD Biosciences, Franklin Lakes, NJ, USA) antibodies for 20 min in the dark. The cells were then washed twice in cold PBS with 1% BSA and resuspended in 300 μl cold PBS with 1% BSA for flow cytometric analysis within 1 h.
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