Nine Wistar rats were randomly divided into the following three groups: (1) blank control (without any interference, BC) group, (2) normal saline injection (NS) group, and (3) carrageenan injection (CAR) group. For injection of carrageenan, rats were anesthetized with an intraperitoneal injection of 4% chloral hydrate (A600288; Sangon Biotech, Shanghai, China) and fixed in a supine position. Then, the lower abdomen above the rats' penis was shaved and the skin in this area was disinfected using three applications of 10% povidone-iodine solution (A606166; Sangon Biotech). A small midline incision was made in the disinfected area, and the bladder and prostate were carefully exposed. Using a microsyringe, 50 μl of sterile suspension of 5% carrageenan suspension (22049; Sigma-Aldrich, St. Louis, MO, USA) was injected into each ventral lobe in the prostate gland.18 (link) A commensurate amount of sterile normal saline was injected in the NS group and nothing was injected in the BC group. After checking for bleeding at the dissection site, the wound was closed in layers.
Carrageenan suspension
Manufactured by Merck Group
Sourced in United States
Carrageenan suspension is a laboratory product used as a thickening and stabilizing agent. It is a natural polymer extracted from red seaweed. The suspension helps to maintain the homogeneity and consistency of various laboratory samples and solutions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using carrageenan suspension
1
Carrageenan-Induced Prostate Inflammation
2
Carrageenan-Induced Rat Paw Edema Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Twelve rats were randomly divided into two groups of 6 rats each (3 males and 3 females). The left hind paw of the rats in the treated group was topically treated with the purified bioglycerol (5%), while paws of those in the control group were treated with 0.9% NaCl solution under the same experimental conditions [48 (link)]. One hour after the application of the tested materials, each rat received a 0.1-ml subplanter injection of 1% carrageenan suspension (Sigma, USA) in the same paw. The thickness of the paw of each rat was measured at 1-, 2-, 3-, and 4-h intervals after the injection of the inflammatory agent. The degree of inflammation was calculated by subtracting the thickness of foot before inflammation from that of the inflamed foot at the different times intervals, and then the inhibition effect of the bioglycerol was calculated according to Lanhers et al. [48 (link)] by the following equation:
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