Lung fibroblasts, to be assessed for cellular proliferation, were cultured either in 96‐well or 48‐well plates. Fibroblast proliferation was determined using colorimetric BrdU incorporation assay kit (Roche) according to manufacturer’s instructions. Absorbance was measured at 370 nm with reference at 492 nm in a plate reader (TECAN). Depending on the experiment, proliferation of cells was plotted either as the difference of absorbance at 370 and 492 nm (A370 nm–A492 nm) or as a percentage of absorbance compared to control cells absorbance.
Colorimetric brdu incorporation assay kit
Manufactured by Roche
Sourced in Germany
The Colorimetric BrdU Incorporation Assay Kit is a laboratory tool used to measure cell proliferation. It detects the incorporation of the synthetic nucleoside bromodeoxyuridine (BrdU) into the DNA of replicating cells. The assay provides a quantitative assessment of BrdU incorporation, which is an indicator of cellular DNA synthesis and proliferation.
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Lab products found in correlation
2 protocols using colorimetric brdu incorporation assay kit
1
Proliferation Assay for Lung Fibroblasts
2
Fibroblast Proliferation via BrdU Assay
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Lung fibroblasts, to be assessed for cellular proliferation, were cultured either in 96‐well or 48‐well plates. Fibroblast proliferation was determined using colorimetric BrdU incorporation assay kit (Roche, Germany) according to manufacturer's instructions. Absorbance was measured at 370 nm with reference at 492 nm in a plate reader (TECAN, Germany). Depending on the experiment, proliferation of cells was plotted either as the difference of absorbance at 370 and 492 nm (A370 nm–A492 nm) or as a percentage of absorbance compared to control cells absorbance.
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