Elisa kits for il 1β

Pseudoephedrine and emodin (purity > 99%) were purchased from Baoji Chenguang biology Ltd. LPS was purchased from Sigma-Aldrich Co. ELISA kits for IL-1β, TNF-α, IL-6, iNOS, IL-10, Arg-1 and VIP were obtained from Elabscience Biotechnology Co. Ltd. (Wuhan, China). Antibodies against AQP-1, AQP-5 VIP, CAMP, PKA/phospho-PKA, IκBα/phospho-IκBα, NF-κB p65/phospho-NF-κB p65, and β-actin were purchased from Affinity Biosciences Ltd. (OH, USA). HRP-conjugated secondary antibodies were purchased from Wuhan Boster Biological Technology LTD. (Wuhan, China). Trizol and Western blot chemiluminescence reagents were obtained from Ambion Thermo Fisher Scientific-CN (Shanghai, China).

Flos Puerariae and Semen Hoveniae were acquired in June from the Jinbei Traditional Chinese Medicine Planting and Breeding Cooperative (Jiangxi, China). DSS reagents were obtained from MP Biomedicals (MP Biomedicals, United States). Enzyme-linked immunosorbent assay (ELISA) kits for IL-1β (lot number E-EL-M0037c), IL-6 (lot number E-EL-M0044c), TNF-α (lot number E-EL-M0049c), and PGE2 (lot number E-EL-M0034c) were obtained from Elabscience (Wuhan, China). Antibodies against PCNA (AH08154078) and CD11b (AB08352478) were purchased from Boster Bio (Pleasanton, United States). Antibodies against inducible nitric oxide synthase (iNOS, 18985-1-AP) and cyclooxygenase-2 (COX-2, 10627-1-BM) were obtained from Abcam (Cambridge, United Kingdom). Antibodies against MAPK pathway proteins were obtained from Cell Signaling Technology (Danvers, MA, United States). HPLC-grade acetonitrile and methanol were obtained from Merck (Darmstadt, Germany). SOD (lot number A001-3-2), MDA (lot number A003-1-2), CAT (lot number A007-1-1), ROS (lot number E004-1-1), and T-AOC (lot number A015-3-1) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).