Avp ires cre

Manufactured by Jackson ImmunoResearch

Avp-IRES-Cre is a genetic construct containing the Avp (Arginine vasopressin) gene promoter, an IRES (Internal Ribosome Entry Site) element, and the Cre recombinase coding sequence. This construct allows for Cre expression under the control of the Avp gene promoter.

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Lab products found in correlation

Teklad f6 rodent diet 8664, by Inotiv (1 mentions) Th cre, by Jackson ImmunoResearch (1 mentions) Agrp ires cre, by Jackson ImmunoResearch (1 mentions) Slc17a6 ires cre, by Jackson ImmunoResearch (1 mentions) Slc32a1 ires cre, by Jackson ImmunoResearch (1 mentions) Nos1 ires cre, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions)

2 protocols using avp ires cre

Housing and Genetic Mouse Models

Cited 2 times

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Animals were housed at 22–24°C on a 12:12 light/dark cycle (light cycle: 6:00 a.m. to 6:00 p.m.) with standard mouse chow (Teklad F6 Rodent Diet 8664; Harlan Teklad) and water provided ad libitum, unless specified otherwise. Adult male and female mice (8–16 weeks old) were used for experiments. Mice were maintained on a mixed background. Transgenic mouse strains used: Avp-IRES-Cre (Pei et al., 2014 (link)), Slc17a6-IRES-Cre (Vglut2-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs Stock 016963), Slc32a1-IRES-Cre (Vgat-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs stock 016962), Nos1-IRES-Cre (Jackson Labs Stock 017526), Slc17a6-IRES-Flp (Vglut2-IRES2-FLPo-D) (Jackson Labs Stock 030212), Slc32a1-IRES-Flp (Vgat-IRES2-FLPo-D) (Jackson Labs Stock 031331), Th-Cre (Savitt et al., 2005 (link)) (Jackson Labs Stock 008601), Dbh-Flp (MMRRC 041575), Avp-GFP (MMRRC 015858-UCD), Agrp-IRES-Cre (Tong et al., 2008 (link)) (Jackson Labs Stock 012899), and Pomc-IRES-Cre (Fenselau et al., 2017 (link)).

Kim A., Madara J.C., Wu C., Andermann M.L, & Lowell B.B. (2021). Neural basis for regulation of vasopressin secretion by anticipated disturbances in osmolality. eLife, 10, e66609.

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Genetically Engineered Mice for Neuroscience

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Adult male Fos^TVA knock-in mice (Sakurai et al., 2016 (link)) were used in this study (available from JAX stock #027831). Adult AVP-IRES-Cre (JAX stock #023530) and C57BL/6 mice were obtained from Jackson Laboratory. For chemogenetic activation of SON AVP neurons, adult male heterozygous AVP^Cre/+ mice were used. For labelling of anesthesia-activated neurons with CANE technology, adult Fos^TVA mice were single-house for two days to quench the background c-fos expression. Subjects were randomly assigned to experimental and control groups. Animals were housed in standard 12-h dark/12-h light cycle at common facility and all experimental procedures were approved by Duke Animal Care and Use Program.

Jiang-Xie L.F., Yin L., Zhao S., Prevosto V., Han B.X., Dzirasa K, & Wang F. (2019). A Common Neuroendocrine Substrate for Diverse General Anesthetics and Sleep. Neuron, 102(5), 1053-1065.e4.

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