An optrode was constructed by gluing four tetrodes to an optical fiber. Four tetrodes spun of 12.7 μm diameter nichrome wire (California Fine Wire) were glued to a 200 μm diameter optical fiber (Thor Labs, SFS200/220Y) and cut so that they extended between 750 and 250 μm beyond the end of the fiber. The tetrodes were pinned into an electrode interface board (EIB; Neuralynx) and the tips were plated by passing 0.2 μA current pulses through the individual wires and a gold solution until the impedance reached 150–200 kOhm. The optrode was mounted on a stereotax arm (Kopf Instruments) and then lowered into the brain during surgery. Two small holes were drilled anterior and posterior to the recording site to serve as sites for ground screws. The ground screws were constructed by soldering stainless steel self-tapping screws to 3 mm stainless steel wire secured to the EIB. Screws were inserted far enough to come in contact with dura.
Stereotax arm
Manufactured by Kopf Instruments
The Stereotax Arm is a precision instrument designed for use in neuroscience research and clinical applications. It provides a stable and accurate platform for the positioning and manipulation of tools or electrodes within the brain. The Stereotax Arm features high-precision adjustments and a range of motion to enable precise targeting of specific brain regions.
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Lab products found in correlation
2 protocols using stereotax arm
1
Optrode Construction for Neural Recording
2
Optrode Construction for Neural Recording
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An optrode was constructed by gluing four tetrodes to an optical fiber. Four tetrodes spun of 12.7 μm diameter nichrome wire (California Fine Wire) were glued to a 200 μm diameter optical fiber (Thor Labs, SFS200/220Y) and cut so that they extended between 750 and 250 μm beyond the end of the fiber. The tetrodes were pinned into an electrode interface board (EIB; Neuralynx) and the tips were plated by passing 0.2 μA current pulses through the individual wires and a gold solution until the impedance reached 150–200 kOhm. The optrode was mounted on a stereotax arm (Kopf Instruments) and then lowered into the brain during surgery. Two small holes were drilled anterior and posterior to the recording site to serve as sites for ground screws. The ground screws were constructed by soldering stainless steel self-tapping screws to 3 mm stainless steel wire secured to the EIB. Screws were inserted far enough to come in contact with dura.
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