The protein expression of Ror2 (105 kDa), FZD5 (65 kDa), CaMKII (50 kDa) β-catenin (86 kDa), cyclin D1 (34 kDa), Wnt5a (45 kDa), GAPDH (37 kDa) and β-actin (43 kDa) were analyzed by western blot analysis using anti-Ror2 rabbit monoclonal (ab92379; Abcam, Cambridge, UK), anti-Frizzled 5 rabbit polyclonal (ab75234; Abcam), anti-CaMKII rabbit polyclonal (sc-13082; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-β-catenin rabbit monoclonal (ab32572; Abcam), anti-cyclin D1 rabbit polyclonal (ab95281; Abcam), anti-Wnt5a rabbit polyclonal (2392; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-GAPDH rabbit monoclonal (5174; Cell Signaling Technology, Inc.) and anti-β-actin rabbit monoclonal (4970; Cell Signaling Technology, Inc.) antibodies according to the manufacturer’s instructions.
Ab92379
Manufactured by Abcam
Sourced in United Kingdom
Ab92379 is a polyclonal antibody that detects the target protein. The antibody is produced in rabbits and purified using protein A chromatography.
Automatically generated - may contain errors
Lab products found in correlation
Ab75234, by Abcam (1 mentions)
Ab95281, by Abcam (1 mentions)
Sc 13082, by Santa Cruz Biotechnology (1 mentions)
Ab32572, by Abcam (1 mentions)
D1306, by Thermo Fisher Scientific (1 mentions)
Prolong diamond antifade mountant media, by Thermo Fisher Scientific (1 mentions)
G2654, by Merck Group (1 mentions)
Ab31419, by Abcam (1 mentions)
Ab39973, by Abcam (1 mentions)
Ab22656, by Abcam (1 mentions)
Ab47308, by Abcam (1 mentions)
A056401 2, by Agilent Technologies (1 mentions)
I2018, by Merck Group (1 mentions)
2 protocols using ab92379
1
Western Blot Analysis of Wnt Pathway Proteins
2
Immunostaining of Pancreatic Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were cultured on glass coverslips and fixed in 2% paraformaldehyde (PFA) for 15 min. The immunofluorescence protocol performed conformed to indications provided by the supplier. The following primary antibodies were used: mouse anti-β-catenin (1/500, ab22656, Abcam), rabbit anti-BMP4 (1/500, ab39973, Abcam), rabbit anti-ROR2 (1/50, ab92379, Abcam), rabbit anti-c-JUN (1/500, ab31419, Abcam), mouse anti-insulin (1/500, I2018, Sigma-Aldrich), guinea-pig anti-porcine insulin (1/500, A056401-2, Dako), mouse anti-porcine glucagon (1/500, G2654, Sigma-Aldrich), rat anti-somatostatin (1/100, sc-47706, Santa Cruz), guinea-pig anti-PDX1 (1/500, ab47308, Abcam), and rabbit anti-NKX6.1 (1/100, NBP1-82553, Novus), rabbit anti-MAFA (1/200, ab98859, Abcam). The following secondary antibodies were used: donkey anti-rabbit A488, donkey anti-rabbit A546, goat anti-mouse A568, chicken anti-rat A647, and goat anti-guinea-pig A647 (1/500, Molecular Probes). The nuclei were stained with DAPI (D1306, Molecular Probes). The samples were mounted in Prolong Diamond Antifade Mountant Media (P36970, Life technologies) and were analyzed with Leica TCS SP5 STED CW confocal microscope. No specific feature of the original data was obscured, eliminated or misrepresented.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!