To measure levels of specific anti Aβ IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA, a similar indirect ELISA protocol was conducted with the addition of specific anti-mouse-immunoglobulin antibodies (ISO-2, Sigma-Aldrich, St. Louis, MO, USA) diluted at 1:1000, incubated for 1 h at RT. Next, donkey anti-goat secondary antibody (705–035-003, Peroxidase AffiniPure Donkey Anti-Goat IgG, Jackson Immunoresearch, PA, USA) diluted at 1:5000 was applied for 1 h at RT. Colorimetric reaction was stopped by adding 50 μl of 2 M H2SO4 solution (339741, Sigma-Aldrich, St. Louis, MO, USA), and OD was measured at 450 nm using a spectrophotometer.
Specific anti mouse immunoglobulin antibodies iso 2
Manufactured by Merck Group
Sourced in United States
Specific anti-mouse-immunoglobulin antibodies (ISO-2) are laboratory reagents used for the detection and quantification of mouse immunoglobulins in various immunoassay techniques. These antibodies recognize and bind to specific epitopes on the immunoglobulin molecules of mice, allowing for the identification and measurement of mouse immunoglobulins in experimental or clinical samples.
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Lab products found in correlation
2 protocols using specific anti mouse immunoglobulin antibodies iso 2
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Anti-Aβ Immunoglobulin ELISA Assay
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Anti-Aβ Immunoglobulin ELISA Assay
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To measure levels of specific anti Aβ IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA, a similar indirect ELISA protocol was conducted with the addition of specific anti-mouse-immunoglobulin antibodies (ISO-2, Sigma-Aldrich, St. Louis, MO, USA) diluted at 1:1000, incubated for 1 h at RT. Next, donkey anti-goat secondary antibody (705–035-003, Peroxidase AffiniPure Donkey Anti-Goat IgG, Jackson Immunoresearch, PA, USA) diluted at 1:5000 was applied for 1 h at RT. Colorimetric reaction was stopped by adding 50 μl of 2 M H2SO4 solution (339741, Sigma-Aldrich, St. Louis, MO, USA), and OD was measured at 450 nm using a spectrophotometer.
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