Stocks of Smith strain MCMV were generated by homogenizing salivary glands harvested from six-week-old BALB/c mice infected with 2.5 x 103 PFU of MCMV at the age of three weeks. Mice were infected at 2 p.m., by the ip injection of 103 PFU/g or 3 x 103 PFU/g (LD50) MCMV diluted in DMEM. “Non-infected” (NI) mice received DMEM only. For PD-1 blockade experiments, 250 μg anti-PD-1 Ab (clone J43 or RMP1-14) or the appropriate isotype control Ab (Armenian Hamster IgG or IgG2a mAb clone 2A3) (all from BioXCell) were injected i.p. into mice on day 1 PI. For IFN-γ neutralization, 500 μg anti-IFNγ Ab (clone XMG1.2) or Rat IgG1 (HRPN) (both from BioXCell) were injected i.p. into mice on day 1 PI. Spleens and livers were harvested after perfusion at different time points, and were processed for FACS or histology analysis, or weighed and homogenized for RNA or protein extraction. Organs were homogenized in a FastPrep-24™ 5G homogenizer (MP Biomedicals).
Anti pd 1 ab clone j43 or rmp1 14
Manufactured by BioXCell
Anti-PD-1 Ab (clone J43 or RMP1-14) is a monoclonal antibody that binds to the programmed cell death-1 (PD-1) receptor. PD-1 is an immune checkpoint molecule that plays a role in regulating T-cell activation and function.
Automatically generated - may contain errors
2 protocols using anti pd 1 ab clone j43 or rmp1 14
1
MCMV Infection and Immunomodulation Protocol
2
MCMV Infection and Immunomodulation Protocol
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Stocks of Smith strain MCMV were generated by homogenizing salivary glands harvested from six-week-old BALB/c mice infected with 2.5 x 103 PFU of MCMV at the age of three weeks. Mice were infected at 2 p.m., by the ip injection of 103 PFU/g or 3 x 103 PFU/g (LD50) MCMV diluted in DMEM. “Non-infected” (NI) mice received DMEM only. For PD-1 blockade experiments, 250 μg anti-PD-1 Ab (clone J43 or RMP1-14) or the appropriate isotype control Ab (Armenian Hamster IgG or IgG2a mAb clone 2A3) (all from BioXCell) were injected i.p. into mice on day 1 PI. For IFN-γ neutralization, 500 μg anti-IFNγ Ab (clone XMG1.2) or Rat IgG1 (HRPN) (both from BioXCell) were injected i.p. into mice on day 1 PI. Spleens and livers were harvested after perfusion at different time points, and were processed for FACS or histology analysis, or weighed and homogenized for RNA or protein extraction. Organs were homogenized in a FastPrep-24™ 5G homogenizer (MP Biomedicals).
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