Anti nanog clone h 155

Manufactured by Santa Cruz Biotechnology

Anti-Nanog (Clone H-155) is a primary antibody developed by Santa Cruz Biotechnology. It is designed to detect Nanog, a transcription factor involved in the maintenance of embryonic stem cell pluripotency.

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Lab products found in correlation

Anti oct3 4 clone h134, by Santa Cruz Biotechnology (2 mentions) Ix83 spinning disk confocal microscope, by Olympus (1 mentions) Anti cd105, by Abcam (1 mentions) Donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti vimentin clonev9, by Merck Group (1 mentions)

Close spelling variants of this product

Nanog (81 citations) Anti-Nanog (37 citations) NANOG (H-155, (2 citations)

2 protocols using anti nanog clone h 155

Immunostaining of pluripotent stem cells

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iPS cells were seeded in chamber slides overnight, and the cells were fixed and permeabilized. Slides were incubated with primary antibodies overnight: anti-Oct3/4 (clone H134, Santa Cruz Biotechnology, Inc. Dallas, TX), anti-Nanog (Clone H-155 Santa Cruz Biotechnology Inc. Dallas, TX), and anti-CD105 (clone 8A1 Abcam, Cambridge, MA). Then washed and incubated with secondary antibodies conjugated to biotin (donkey anti mouse IgG or donkey anti rabbit IgG; Jackson ImmunoResearch Laboratories, Inc. West Grove, PA) and counter stained with DAPI. Visualization of florescence staining was performed on Olympus IX83 spinning disk confocal microscope.

Chow L., Johnson V., Regan D., Wheat W., Webb S., Koch P, & Dow S. (2017). Safety and immune regulatory properties of canine induced pluripotent stem cell-derived mesenchymal stem cells. Stem cell research, 25, 221-232.

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NPC Immunostaining: Detailed Protocol

Cited 1 time

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NPC immunostaining was performed according to previously published protocols (26 (link), 29 (link)). Primary antibodies used were anti-Nestin (clone 10C2; Merck Millipore, Billerica, MA), anti-TUJ1 (beta III tubulin; clone TUJ1 Covance Inc. San Diego, CA), anti-Vimentin (clone V9; Merck Millipore, Billerica, MA), anti-Oct3/4 (clone H134, Santa Cruz Biotechnology, Inc. Dallas, TX), anti-Nanog (Clone H-155 Santa Cruz Biotechnology Inc. Dallas, TX).

Chow L., McGrath S., de Arruda Saldanha C., Whalen L.R., Packer R, & Dow S. (2020). Generation of Neural Progenitor Cells From Canine Induced Pluripotent Stem Cells and Preliminary Safety Test in Dogs With Spontaneous Spinal Cord Injuries. Frontiers in Veterinary Science, 7, 575938.

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