A 5 μL aliquot of the diluted extract was separated on an Agilent Zorbax Eclipse Plus C18 column (2.1 × 100 mm, 3.5 μm) using a step/ramp gradient starting from 2:98 methanol:10 mM ammonium formate (pH 3.0) to 50% acetonitrile. The flow from the HPLC (Agilent 1200 or 1260 Infinity) was directed to a Sciex 5500 QTrap triple quadrupole mass spectrometer. Detailed analytical parameters for LC and MS acquisition are available in Data S1.
1260 infinity
Manufactured by AB Sciex
Sourced in United States, Germany
The 1260 Infinity is a liquid chromatography system designed for various analytical applications. It features a modular design, allowing for customization to meet specific laboratory needs. The system includes a high-performance pump, autosampler, column compartment, and diode array detector, providing reliable and precise liquid chromatography performance.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 1260 infinity
1
HPLC-MS/MS Metabolite Analysis
2
Automated Cannabinoid Analysis Pipeline
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the general chromatography, 5 μl of the extract was separated in 13 min on an Agilent Zorbax Eclipse Plus C18 (2.1 × 100 mm, 3.5 μm) maintained at 50 • C. Mobile phase A was an aqueous solution of ammonium formate/formic acid (pH 3.0):methanol (98:2). Mobile phase B was methanol (EMD Millipore corporation, MX0486-1, Billerica, MA, USA). A 650 μl step/ramp from the A to the B mobile phase was used. Detailed gradient, analyte retention times, Q1/Q3 identification and confirmation transitions, source and mass spectrometer parameters are available in Supplementary Data 1.
For the cannabinoid chromatography, a 10 μl aliquot of the extract was separated using the above conditions, except the column was 50 mm long and a 550 μl/min flow rate was used for the 6.5 min separation. Analytical details are available in Supplementary Data 1.
In both cases, an Agilent HPLC 1200 or 1260 Infinity coupled to a Sciex 5500 QTrap mass spectrometer were used. The data acquisition software used was Analyst1 1.6.2 build 8489. Data was analyzed using Multiquant1 3.0.1 (Version 3.0.6256.0) software.
For the cannabinoid chromatography, a 10 μl aliquot of the extract was separated using the above conditions, except the column was 50 mm long and a 550 μl/min flow rate was used for the 6.5 min separation. Analytical details are available in Supplementary Data 1.
In both cases, an Agilent HPLC 1200 or 1260 Infinity coupled to a Sciex 5500 QTrap mass spectrometer were used. The data acquisition software used was Analyst1 1.6.2 build 8489. Data was analyzed using Multiquant1 3.0.1 (Version 3.0.6256.0) software.
3
Profiling Phenolic Compounds via LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The profile of phenolic compounds was analyzed using an Agilent model 1260 Infinity (Palo Alto, CA, USA) connected directly to a mass detector AB SCIEX Triple Quad 3500 (AB Sciex, Foster City, CA, USA) equipped with a turbo V™ electrospray ionization source (ESI), and to a Waters 996 photodiode array detector. The separation was carried out on a Phenomenex Luna C18 column (150 mm × 2 mm; 3 μm), with an injection volume of 5 μL and a flow of 300 μL/min. The mobile phase was 0.1% (v/v) formic acid in water (A) and 0.1% (v/v) formic acid in acetonitrile (B). The gradient used was: 98% A (v/v), 0–4 min; 98–80% A (v/v), 4–7 min; 80–10% A (v/v), 7–14 min; 10% A (v/v), 14–15 min; 10–98% A (v/v), 15–17 min. The mass spectrometer was run in positive and negative ionization, using N2 as the nebulizer and collision gas, with an ion spray voltage of 4500 V, source temperature of 400 °C and nebulizer gas pressure of 55 psi. The compounds were identified by the comparison with the corresponding standards, based on retention time data, extracted ion chromatograms and MS/MS spectra. For the quantification, calibration curves were made by using different concentrations of the standards and plotting them against peak areas.
4
Quantification and Bioaccumulation of Ibuprofen in Plants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extraction, separation, concentration, and validation of IBU from experiment samples were analyzed according to Picó et al. [26 (link)] and Andreotti et al. [64 (link)] using ultra-high performance liquid chromatography (Agilent 1260 Infinity, Waldbronn, Germany) and an AB SCIEX TripleTOF™ 5600 mass spectrometer (AB SCIEX, Foster City, CA, USA). Data acquisition processing and instrument control were performed using Analyst, Peak View 1.0, and MultiQuant 2.0. software [26 (link)].
The bioconcentration factor (BCF), bioaccumulation factor (BAF), and translocation factor (TF) of IBU in V. unguiculata plants were calculated according to Wang [65 ]:
The bioconcentration factor (BCF), bioaccumulation factor (BAF), and translocation factor (TF) of IBU in V. unguiculata plants were calculated according to Wang [65 ]:
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!