Human breast cancer cell lines MDA361, T47D, SKBR3, MDA-MB-468 (MDA-468), MCF-12A, and MCF-10A were purchased from American Type Culture Collection (ATCC). MDA361, T47D, MDA-468, and SKBR3 were cultured in DMEM/F-12 (Mediatech Inc.) supplemented with 10% FBS and penicillin/streptomycin. HMLE (kindly provided by Dr. R. A. Weinberg) cell lines were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 medium (Mediatech Inc.) supplemented with 5% FBS (Clontech), 100 units/ml penicillin-streptomycin (Invitrogen), 2 mml-glutamine (Invitrogen), 10 ng/ml human epidermal growth factor (EGF) (Invitrogen), 0.5 μg/ml hydrocortisone (Sigma), and 10 μg/ml insulin (Sigma).MCF-12A and MCF-10Awere cultured in 1:1 Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 medium (Mediatech Inc.) supplemented with 5% Horse serum (Clontech), 100 units/ml penicillin-streptomycin, 10 ng/ml human epidermal growth factor (EGF), 0.5 μg/ml hydrocortisone, and 10 μg/ml insulin .
Dmem ham s f 12 medium
Manufactured by Corning
Sourced in United States
DMEM/Ham's F-12 medium is a cell culture medium used for the growth and maintenance of a variety of cell types. It is a mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 nutrient mixture, providing essential nutrients, vitamins, and amino acids required for cell proliferation and survival.
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Lab products found in correlation
F 12k medium, by Corning (2 mentions)
Sv huc 1, by Corning (2 mentions)
Sv huc 1, by American Type Culture Collection (2 mentions)
Hydrocortisone, by Takara Bio (2 mentions)
Insulin, by Takara Bio (2 mentions)
Penicillin streptomycin, by Takara Bio (2 mentions)
Glutamine, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Takara Bio (2 mentions)
Mcf 12a, by Merck Group (2 mentions)
Horse serum, by Takara Bio (2 mentions)
Human epidermal growth factor egf, by Takara Bio (2 mentions)
Human epidermal growth factor, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Insulin, by Merck Group (2 mentions)
Hydrocortisone, by Merck Group (2 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Mda 361, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Penicillin, by GE Healthcare (1 mentions)
5 protocols using dmem ham s f 12 medium
1
Culture and Maintenance of Breast Cancer Cell Lines
2
Culture and Maintenance of Breast Cancer Cell Lines
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Human breast cancer cell lines MDA361, T47D, SKBR3, MDA-MB-468 (MDA-468), MCF-12A, and MCF-10A were purchased from American Type Culture Collection (ATCC). MDA361, T47D, MDA-468, and SKBR3 were cultured in DMEM/F-12 (Mediatech Inc.) supplemented with 10% FBS and penicillin/streptomycin. HMLE (kindly provided by Dr. R. A. Weinberg) cell lines were cultured in 1:1 Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 medium (Mediatech Inc.) supplemented with 5% FBS (Clontech), 100 units/ml penicillin-streptomycin (Invitrogen), 2 mml-glutamine (Invitrogen), 10 ng/ml human epidermal growth factor (EGF) (Invitrogen), 0.5 μg/ml hydrocortisone (Sigma), and 10 μg/ml insulin (Sigma).MCF-12A and MCF-10Awere cultured in 1:1 Dulbecco’s Modified Eagle’s Medium (DMEM)/Ham’s F-12 medium (Mediatech Inc.) supplemented with 5% Horse serum (Clontech), 100 units/ml penicillin-streptomycin, 10 ng/ml human epidermal growth factor (EGF), 0.5 μg/ml hydrocortisone, and 10 μg/ml insulin .
3
Cell Line Cultivation and Authentication
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Human normal uroepithelial (SV-HUC-1), human transitional bladder cancer (T24) and human grade II urinary carcinoma (5637) cell lines were purchased from American Type Culture Collection (ATCC, USA). T24 cells were cultured in DMEM/Ham`s F-12 medium (Corning, USA) with 10% foetal bovine serum (FBS) supplemented with 5 μg/ml amphotericin B, 100 μg/ml streptomycin and 100 U/ml penicillin. SV-HUC-1 cells were cultured in F-12 K medium (Corning, USA) with same supplements. 5637 cells were cultured in RPMI-1640 medium (Corning, USA) with same supplements. All cell lines were grown in plastic tissue culture T-flasks 75 cm2 (Corning, USA) at 37 °C and 5% CO2. Cell lines were authenticated by American Type Culture Collection.
4
Cell Culture Conditions for Cancer Research
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SV-HUC-1 (non-malignant human urothelium), T24 (human bladder cancer), DU-145 (human prostate cancer), and RWPE-1 (non-malignant human prostate epithelium) cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). T24 and DU-145 were cultured in DMEM/Ham’s F-12 medium (Corning, New York, NY, USA) containing 10% fetal bovine serum (FBS, Pan-Biotech GmbH, Aidenbach, Germany) supplemented with 5 μg/mL of amphotericin B (GE Healthcare Life Science, Chicago, IL, USA), 100 μg/mL of streptomycin, and 100 U/mL of penicillin (GE Healthcare Life Science, Chicago, IL, USA). SV-HUC-1 was cultured in F-12K medium (Corning, New York, NY, USA) with the same supplementation. RWPE-1 was cultured in KBM-Gold™ Keratinocyte Cell Basic Medium (Lonza, Basel, Switzerland) supplemented with a KGM-Gold SingleQuot Kit (Lonza, Basel, Switzerland). All cell lines were grown in a plastic tissue culture 75 cm2 T-flasks (Corning, New York, NY, USA) at 37 °C and 5% CO2.
5
Differentiation of SH-SY5Y Cells
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SH-SY5Y (ATCC; REF: CRL-2266) cells were cultivated in DMEM/Ham's F-12 medium (Corning) supplemented with Fetal Bovine Serum (Corning) and penicillin/streptomycin (DMEM/Ham's F-12/FBS/pen-strep) at 37 o C/5 % CO 2 under constant humidity. Upon reaching a 40 % confluency, the cells were replated/split to 8 × 15 cm cell culture dishes at an initial seeding density of 8000 cells/cm 2 and cultivated in DMEM/Ham's F-12/FBS/pen-strep as described above. Upon reaching a 20 % -30 % confluency, the culture medium of the cells was replaced with Neurobasal medium (Gibco) supplemented with glutamax (Gibco), B27 supplement (Gibco) and 15 μM all-trans-retinoic acid (ATRA) and the cultivation continued for 5 days. The cells were harvested for RNA granule isolation as described in "RNA Granule isolation".
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