Wild type CHO (ATCC, Manassas, Virginia, U.S.) and a serine-SPT deficient CHO cell line, known as LY-B8 (link) (RIKEN BioResource Research Center, Koyadai, Japan), were used. Cell culture products; DMEM:F12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12) FBS (Fetal Bovine Serum), penicillin, streptomycin, and GlutaMax suplement were purchased from Thermofisher (Waltham, MA). Organic solvents were from Thermofisher (Waltham, MA). All fluorophores; were purchased from Thermofisher (Waltham, MA). Salts for buffer preparation (KCl, NaCl, CaCl2, HEPES), myriocin, L-α-phosphatidylcholine from chicken egg (PC), sphingomyelin from chicken egg (SM) and D-erythro-sphinganine (sphinganine) were purchased from Sigma-Aldrich (St. Louis, MO, U. S.). All other reagents (salts and Organic solvents) were of analytical grade.
Ly b8
Manufactured by RIKEN BioResource Center
Sourced in United States, Japan
The LY-B8 is a laboratory equipment designed for cell culture applications. It is a benchtop incubator that provides a controlled environment for the growth and maintenance of cells. The LY-B8 maintains temperature, humidity, and carbon dioxide levels within optimal ranges to support cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Hepes, by Merck Group (2 mentions)
Cacl2, by Merck Group (2 mentions)
Wild type cho, by American Type Culture Collection (2 mentions)
Myriocin, by Merck Group (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Organic solvents, by Thermo Fisher Scientific (1 mentions)
Glutamax suplement, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using ly b8
1
Serine-SPT Deficient CHO Cell Line
2
Serine-SPT Deficient CHO Cell Line
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Wild type CHO (ATCC, Manassas, VA, US) and a serine-SPT de cient CHO cell line, known as LY-B 8 (RIKEN BioResource Research Center, Koyadai, Japan), were used. Cell culture products: DMEM:F12 (Dulbecco's Modi ed Eagle Medium: Nutrient Mixture F-12), penicillin, streptomycin, and GlutaMax supplement were purchased from Thermo sher (Waltham, MA, US). Fetal bovine serum (FBS) was lot no. 2176377 from Gibco (Dublin, Ireland). Organic solvents were from Thermo sher (Waltham, MA, US). All uorophores were purchased from Thermo sher (Waltham, MA, US). Salts for buffer preparation (KCl, NaCl, CaCl 2, HEPES), myriocin and lipids were purchased from Sigma Aldrich (Sigma Aldrich, San Luis, MO, US). All reagents (salts and organic solvents) were of analytical grade.
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