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2 protocols using amino antipyrine

1

Gentamicin Injection Quality Assessment

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Commercial Gentamicin injection (80 mg) was collected from a model pharmacy (Dhaka, Bangladesh). Laboratory standard Thiobarbituric acid, Trichloroacetic acid, Hydrochloric acid, 10% formalin, 0.9% saline, Phosphate buffer, Detergent, Uricase, Dichlorophenol sulphonate, Ascorbate oxidase, Peroxidase, Amino-antipyrine, Urease Enzyme reagent, EDTA, Sodium salicylate, Sodium nitroprusside, Alkaline hypochlorite, Sodium hypochlorite, Sodium hydroxide, Urea standard, Picric acid, Creatinine Standard, Nitrite standard solution, Griess reagent, and Alcohol were purchased from Sigma Aldrich (St. Louis, MO, USA) and used during the research.
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2

Quantification of Amine Oxidase Activity

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Aminoantipyrine (AAP), ammonia assay kit, bovine serum albumin (BSA), broad range SDS-PAGE molecular weight standards, concanavalin-A coupled with fluorophore fluorescein isothiocyanate (Con-A-FITC), desloratadine, 3,5-dichloro-2-hydroxybenzoic acid (DCHBS), histamine, hydrogen peroxide (H2O2), homovanillic acid (HVA), paraformaldehyde, horseradish peroxidase (HRP) type II, HRP type X, pig kidney diamine oxidase (pkDAO), protease inhibitor cocktail, putrescine, pyridoxal 5′-phosphate (PLP), semicarbazide and N-nitro-L-arginine methyl ester (L-NAME) were purchased from Sigma-Aldrich (Oakville, Ontario, Canada). Bradford reagent, acrylamide/bis-acrylamide solution (29:1) and Precision plus Protein Kaleidoscope Standards were from Bio-Rad Laboratory (Mississauga, Ontario, Canada). All current chemicals were Reagent Grade and were used without further purification.
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