Cell precipitation was suspended and washed with PBS supplemented with 2%BSA, and then the cells were stained with the anti-CD90 primary antibody (Abcam, Cambridge, UK) and anti-CD45 primary antibody (Abcam, Cambridge, UK) for 30 min in 4 °C.The isotype was used as a negative control. Ultimately, the samples were sorted by DAKO Flow Cytometer (DAKO, CA, USA).
Dako flow cytometer
Manufactured by Agilent Technologies
Sourced in United States, Germany
The DAKO Flow Cytometer is a high-performance laboratory instrument designed for the analysis of cells and particles in suspension. It utilizes the principles of flow cytometry to detect and measure various cellular characteristics, such as size, granularity, and fluorescence intensity. The instrument is capable of rapidly analyzing multiple parameters on individual cells within a sample, providing detailed insights into cellular composition and function.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dako flow cytometer
1
Cell Surface Marker Profiling
2
Identification of Mouse Regulatory T Cells
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Mouse Regulatory T Cell Staining Kit was used to check the FoxP3+CD4+CD25+ regulatory T (FoxP3+Treg) cells according to manufacturer's instructions. Briefly, 100 µl of freshly prepared splenocytes (1×106cells) were stained with anti-mouse CD4 and anti-mouse CD25 for 30 min at 4°C. After staining surface markers, cells were incubated with fixation/permeabilization working solution for 30 min at 4°C. Then, the cells were washed with permeablilization buffer and stained with anti-mouse Foxp3 antibody or isotype control at 40 for at least 30 min in dark. The cells were then washed with permeabilization buffer and suspended in flow cytometry staining buffer. All samples were analyzed on DAKO flow cytometer (Partec, Germany), using Cyflogic software (version: 1.2.1).
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